Dataset Information

Proteomics analysis of humulene-producing yeast integrated with a low temeprature-inducible GAL circuit for pathway induction

ABSTRACT: We analysed the proteome of two humulene-producing yeast strains under normal temperature (30 °C) cultivation condition and low temperature (25 °C) cultivation condition. In these two humulene-producing yeast strains, HUM401RU (control strain) and CI-HUM401RU (low-temperature-inducible strain), the mevalonate pathway genes and the humulene synthase gene are controlled by different galactose-inducible (GAL) promoters. For CI-HUM401RU, a low-temperature-inducible GAL circuit has been introduced to enable induction of pathway genes at 25 °C. For the control strain, HUM401RU, a tetracycline-inducible promoter is used to control GAL80, so that the pathway genes can be auto-induced after glucose depleted during the cultivation. We in total submitted 12 samples which can be devided into four groups: (1) HUM401RU, 25 °C, 24h; (2) CI-HUM401RU, 30 °C, 24h; (3) CI-HUM401RU, 25 °C, 24 h; (4) CI-HUM401RU, 25 °C, 48 h. Each group has three bilogical replites.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) Saccharomyces Cerevisiae

SUBMITTER: Zeyu Lu

LAB HEAD: Zeyu Lu

PROVIDER: PXD047054 | Pride | 2024-08-10

REPOSITORIES: Pride

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LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae.

Lu Zeyu Z Shen Qianyi Q Bandari Naga Chandra NC Evans Samuel S McDonnell Liam L Liu Lian L Jin Wanli W Luna-Flores Carlos Horacio CH Collier Thomas T Talbo Gert G McCubbin Tim T Esquirol Lygie L Myers Chris C Trau Matt M Dumsday Geoff G Speight Robert R Howard Christopher B CB Vickers Claudia E CE Peng Bingyin B

Nucleic acids research 20240701 12

Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) in the model yeast Saccharomyces cerevisiae. Two temperature biosensors, a heat-inducible degron and a heat-inducible protein aggregation domain, were used to regulate the GAL activator Gal4p, rendering the leaky LowTempGAL systems. Boolean-type induction was achieved by implementing a second-layer control ...[more]

PMID: 38808673

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