Proteomics

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Propagation of a rapid cell-to-cell H2O2 signal over long distances in a monolayer of cardiomyocyte cells


ABSTRACT: Cell-to-cell communication plays a cardinal role in the biology of multicellular organisms. H2O2 is an important cell-to-cell signaling molecule involved in the response of mammalian cells to wounding and other stimuli. We previously identified a signaling pathway that transmits wound-induced H2O2 cell-to-cell signals within minutes over long distances, measured in centimeters, in a monolayer of cardiomyocyte cells. Here we report that this long-distance H2O2 signaling pathway is accompanied by enhanced accumulation of cytosolic H2O2 and altered redox state in cells along its path. In addition, we show that it requires the production of superoxide, as well as the function of gap junctions, and that it is accompanied by significant changes in the abundance of hundreds of proteins in different cells along its path. Our findings highlight the existence of a unique rapid long-distance H2O2-dependent signaling pathway that could play an important role in different inflammatory responses, wound responses/healing, cardiovascular disease, and/or other diseases

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture

SUBMITTER: Brian Mooney  

LAB HEAD: Ron Mittler

PROVIDER: PXD047131 | Pride | 2024-05-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Linda_Mittler_directDIA_rat_local.sne Other
Linda_Mittler_directDIA_rat_sytemic_injured.sne Other
N10_Systemic_uninjured_3SUW_2717.d.zip Other
N11_local_injured_4LW_2718.d.zip Other
N12_local_uninjured_4LUW_2719.d.zip Other
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Publications

Propagation of a rapid cell-to-cell H<sub>2</sub>O<sub>2</sub> signal over long distances in a monolayer of cardiomyocyte cells.

Fichman Yosef Y   Rowland Linda L   Nguyen Thi Thao TT   Chen Shi-Jie SJ   Mittler Ron R  

Redox biology 20240209


Cell-to-cell communication plays a cardinal role in the biology of multicellular organisms. H<sub>2</sub>O<sub>2</sub> is an important cell-to-cell signaling molecule involved in the response of mammalian cells to wounding and other stimuli. We previously identified a signaling pathway that transmits wound-induced cell-to-cell H<sub>2</sub>O<sub>2</sub> signals within minutes over long distances, measured in centimeters, in a monolayer of cardiomyocytes. Here we report that this long-distance H<  ...[more]

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