Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ORF1p interactors in the mouse brain

ABSTRACT: Mass spectrometry analysis of endogenous LINE-1 encoded ORF1p interactors in the mouse brain.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Vanessa Masson

LAB HEAD: Damarys Loew

PROVIDER: PXD047160 | Pride | 2024-09-10

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Design_PRIDE.xlsx	Xlsx
	H5138BL.msf	Msf
	H5138BL.raw	Raw
	H5139BL.msf	Msf
	H5139BL.raw	Raw

Items per page:

1 - 5 of 21

Similar Datasets

Role of Astrocytes in critical periods

Project description:Brain postnatal development is characterized by critical periods of experience dependent remodeling. Maturation of local circuits inhibitory neurons terminate this period of enhanced plasticity. Astroglial cells are known to influence excitatory and inhibitory synaptic transmission as well as network activity through active signaling mechanisms. Although these can be developmentally regulated, the role of astrocytes in the timing of post-natal critical period is unknown. Here we show in the visual cortex that astrocytes con-trol the maturation of inhibitory neurons and thereby closure of the critical period. We uncover a novel underlying pathway involving regulation of the extracellular matrix that allows interneurons maturation via astroglial connexin signaling. We find that timing of the critical period closure is controlled by a marked upregulation of the astroglial protein connexin 30 that inhibits expression of the matrix degrading enzyme MMP9 through the RhoA-GTPase pathway. Our results thus demonstrate that astrocytes not only influ-ence neuronal activity but are also key elements in the experience–dependent wiring of brain circuits. Therefore, astrocytes represent a new cellular partner to consider in our understanding of the post-natal shaping of neuronal activities, hence providing a new target to alleviate malfunctions associated to im-paired closure of the critical period and settling of synaptic circuits.

2022-05-12 | PXD020448 | Pride

Translation regulation mechanisms in astrocytes

Project description:Identifying the proteins associated with the polyribosomes in astrocytes from the mouse brain to unveil translation regulation mechanisms in these cells.

2023-07-20 | PXD033121 | Pride

ChIPseq of DDX3X, DDX54, ERalpha and H3K4me3 in human breast cancer MCF7 cells

Project description:We aimed to investigate the chromatin binding activity of DDX3X and DDX54 RNA helicases in human ER -dependent breast cancer MCF7 cells. We run a parallel chromatin binding profiling of ER ChIP-seq. H3K4me3 profiling was used as a quality control of the ChIP-seq procedure.

2019-11-14 | E-MTAB-6211 | biostudies-arrayexpress

RagG interactome analysis in human breast MDA-MB-231 cells

Project description:RagC was immunoprecipitated from human breast cancer MDA-MB-231 cells treated or not with the Arp2/3 complex inhibitor, CK666, and the RagC interactome was analyzed by mass-spectrometry.

2023-09-04 | PXD027100 | Pride

KDM5 Lysine Demethylases Are Involved in Maintenance of 3'UTR Length [ChIP-Seq]

Project description:We discovered that the Saccharomyces cerevisiae lysine demethylase, Jhd2 (also known as KDM5), recruits 3'UTR processing machinery and promotes alteration of 3'UTR length in a demethylase-dependent manner. Interaction of Jhd2 with both chromatin and RNA suggests that Jhd2 affects selection of polyadenylation sites through a transcription-coupled mechanism. Wild-type yeast or yeast with JHD2 deleted were grown to mid-log phase. ChIP analysis performed for H3K4me3, Pol2, IgG.

2016-03-23 | E-GEOD-67211 | biostudies-arrayexpress

Identification of CEBPA and RUNX1 interactors in 3q-rearranged AML (MOLM-1)

Project description:Chromatin immunoprecipitation with specific isolation of chromatin associated proteins (ChIP-SICAP) was used to identify chromatin-bound interactors of CEBPA and RUNX1 in the 3q-rearranged human myeloid leukemia cell line MOLM-1.

2021-04-29 | PXD020050 | Pride

Identification of CEBPA and RUNX1 interactors in 3q-rearranged AML (HNT-34)

2021-04-29 | PXD020054 | Pride

Identification of CEBPA and RUNX1 interactors in 3q-rearranged AML

2021-04-29 | PXD020045 | Pride

ChIP-seq experiment to determine the genomic binding sites of mutant V5-tagged ZFHX2 R1913K protein in a stable-expressing human SH-SY5Y neuroblastoma cell line

Project description:A novel missense mutation has been identified in a human pain-insensitive family in the gene ZFHX2. We modelled this mutation in mice by changing the orthologous amino acid and carried out pain behaviour tests in mutant BAC transgenic mice. These showed a phenotype of hyposensitivity to noxious thermal stimuli. In this experiment, we generated a stable cell line expressing the mutant human ZFHX2 protein in order to identify chromatin binding sites. Complementary microarray data from ZFHX2 mutant mice were also deposited at ArrayExpress under accession number E-MTAB-5650 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5650 ).

2018-06-11 | E-MTAB-5651 | biostudies-arrayexpress