Proteomics

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The synaptic proteome and phosphoproteome is uniquely vulnerable to sleep loss during brain development while maturation confers resilience

ABSTRACT: Sleep supports lifelong brain health and cognition. Sleep loss in early life can drive lasting changes in adult behavior, indicating sleep plays a distinct but poorly understood role supporting brain development. We systematically examined the molecular and behavioral adaptations and synaptic consequences of acute sleep deprivation (SD) in developing and adult mice. Developing mice lack robust adaptations to SD, exacerbating cognitive deficits. Synapse proteome and phosphoproteome analysis revealed profound vulnerability to SD in developing mice, including immediate impacts on synaptogenesis and key aspects of brain development. With maturation, a unified biochemical effect of sleep on synapses emerges, together with robust adaptations and resilience to SD. Our findings show sleep plays a distinct role in early life supporting synapse development, transitioning to homeostatic functions with maturation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Christine Mills  

LAB HEAD: Graham Diering

PROVIDER: PXD047381 | Pride | 2024-10-08

REPOSITORIES: Pride

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Developing forebrain synapses are uniquely vulnerable to sleep loss.

Gay Sean M SM   Chartampila Elissavet E   Lord Julia S JS   Grizzard Sawyer S   Maisashvili Tekla T   Ye Michael M   Barker Natalie K NK   Mordant Angie L AL   Mills C Allie CA   Herring Laura E LE   Diering Graham H GH  

bioRxiv : the preprint server for biology 20240412

Sleep is an essential behavior that supports lifelong brain health and cognition. Neuronal synapses are a major target for restorative sleep function and a locus of dysfunction in response to sleep deprivation (SD). Synapse density is highly dynamic during development, becoming stabilized with maturation to adulthood, suggesting sleep exerts distinct synaptic functions between development and adulthood. Importantly, problems with sleep are common in neurodevelopmental disorders including autism  ...[more]

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