Proteomics

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Phenotypic and Proteomic Insights into Differential Cadmium Accumulation in Maize Kenerls


ABSTRACT: The contamination of agricultural soil by heavy metal cadmium (Cd) poses a significant environmental challenge, affecting crop growth, development and, human health. Previous studies have established the pivotal role of the ZmHMA3 gene, a P-type ATPase heavy metal transporter, in determining variable Cd accumulation in maize grains among 513 inbred lines. To decipher the molecular mechanism underlying mutation-induced phenotypic differences mediated by ZmHMA3, we conducted a quantitative Tandem Mass Tag (TMT)-based proteomic analysis of immature maize kernels. This analysis aimed to identify differentially expressed proteins (DEPs) in wild-type B73 and zmhma3 null mutant under Cd stress. The findings demonstrated that zhma3 accumulated higher levels of Cd compared to B73 when exposed to varying Cd concentrations in the soil. In comparison to low Cd concentration soil, B73 and zmhma3 exhibited 75 and 142 DEPs, respectively, with 24 common DEPs shared between them. Zmhma3 showed a higher induction of upregulated genes related to Cd stres than B73. Amino sugar and nucleotide sugar metabolism were specifically enriched in B73, while phenylpropanoid biosynthesis, nitrogen metabolism, and glyoxylate and dicarboxylate metabolism appeared to play a more significant role in zmhma3. This study provides proteomics insights into unraveling the molecular mechanism underlying the differences in Cd accumulation in maize kernel.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Embryo

SUBMITTER: Su Tian  

LAB HEAD: Bin Tang

PROVIDER: PXD047578 | Pride | 2024-01-26

REPOSITORIES: Pride

Dataset's files

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Action DRS
WD400TQ01_1.raw Raw
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Publications

Phenotypic and Proteomic Insights into Differential Cadmium Accumulation in Maize Kernels.

Guo Huanle H   Deng Min M   Yu Feng F   Li Han H   Cao Zhongyang Z   Zeng Qiang Q   Chen Zhihui Z   Luo Hongbing H   Tang Bin B  

Genes 20231213 12


The contamination of agricultural soil with cadmium (Cd), a heavy metal, poses a significant environmental challenge, affecting crop growth, development, and human health. Previous studies have established the pivotal role of the <i>ZmHMA3</i> gene, a P-type ATPase heavy metal transporter, in determining variable Cd accumulation in maize grains among 513 inbred lines. To decipher the molecular mechanism underlying mutation-induced phenotypic differences mediated by <i>ZmHMA3</i>, we conducted a  ...[more]

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