FERONIA-mediated CC1 phosphorylation dynamically regulates cortical microtubule behavior and salt stress responses in Arabidopsis

ABSTRACT: Cell wall remodeling is important for plants to adapt to environmental stress, and therefore needs to be efficiently modulated during stress conditions. Under salt stress, cortical microtubules undergo a depolymerization-reassembly process to promote the biosynthesis of stress-adaptive cellulose, but the regulatory mechanisms underlying this process are still largely unknown. In this study, we reveal that FERONIA, a potential cell wall sensor, interacts with CC1 and its closest homolog, CC2; two proteins that are required for cortical microtubule reassembly under salt stress. Biochemical data indicate that FER phosphorylates CC1 on multiple residues in the second and third hydrophobic microtubule-binding regions in vitro and in vivo, and that these phosphorylations impact the ability of CC1 to engage with microtubules. Furthermore, FER kinase activity and the CC1 phosphorylation level were temporally coordinated upon exposure to salt stress, which coincided with dynamic microtubule reorganization. Both fer-4 and cc1 cc2 mutants displayed similar salt-sensitive phenotypes, which were related to compromised microtubule reassembly, corroborating that FER and CC1/CC2 function in the same pathway to regulate microtubule organization. Taken together, our study outlines an important intracellular mechanism that maintains microtubule dynamics during salt exposure in plant cells.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Chunzhao Zhao  

LAB HEAD: Chunzhao Zhao

PROVIDER: PXD047608 | Pride | 2024-08-30

REPOSITORIES: pride