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DiGly_Analysis_RAD18_RAD6_with/without_MAGEA4

ABSTRACT: Auto-ubiquitination assay performed in which the E3 ligase complex RAD18-RAD6 was incubated with E1 and ubiquitin in the presence/absence of MAGEA4for 1 hour at 37C. All proteins were purified from E.coli. Samples were loaded and run onto a precast SDS PAGE gel, which was stained with Coomasie. Approximately 20ug of RAD18-RAD6 was used for the assay.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Frank Stein

LAB HEAD: Sagar Bhogaraju

PROVIDER: PXD047850 | Pride | 2024-02-20

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	F117190.dat	Other
	F117191.dat	Other
	F117192.dat	Other
	F117193.dat	Other
	P2307_Zelda_221014_P2307_JS_SGJ_T_B1_R2_results_20221103_1422_peptides.txt	Txt

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