Dataset Information

LC-MS/MS data for HCA mRNA antibodies

ABSTRACT: Fc N-glycosylation of IgGs is necessary for effector functions and is a crucial component of quality control. Fc N-glycan profiles of HCA produced by two platforms were determined using mass spectrometry which revealed major differences in site occupancy, glycan types, and glycoform distribution.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Chaoshuang Xia

LAB HEAD: Catherine E. Costello

PROVIDER: PXD047910 | Pride | 2024-05-22

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	090921_HCA_N_IgG.raw	Raw
	090921_HCA_N_IgG.xlsx	Xlsx
	20220701_HCA_mRNA_VK2_IgG.raw	Raw
	20220701_HCA_mRNA_VK2_IgG.xlsx	Xlsx
	20230802_HCA_N_IgG_18O_Deglycosylated.raw	Raw

Items per page:

1 - 5 of 9

Publications

Platform-Specific Fc <i>N</i>-Glycan Profiles of an Antisperm Antibody.

Nador Ellena E Xia Chaoshuang C Santangelo Philip J PJ Whaley Kevin J KJ Costello Catherine E CE Anderson Deborah J DJ

Antibodies (Basel, Switzerland) 20240306 1

IgG Fc <i>N</i>-glycosylation is necessary for effector functions and is an important component of quality control. The choice of antibody manufacturing platform has the potential to significantly influence the Fc glycans of an antibody and consequently alter their activity and clinical profile. The Human Contraception Antibody (HCA) is an IgG1 antisperm monoclonal antibody (mAb) currently in clinical development as a novel, non-hormonal contraceptive. Part of its development is selecting a suit ...[more]

PMID: 38534207

Similar Datasets

Project description:Melanoma is an invasive malignancy with a high frequency of blood-borne metastases, but circulating tumor cells (CTCs) have not been readily isolated. We adapted microfluidic CTC capture to a tamoxifen-driven B-RAF/PTEN mouse melanoma model. CTCs were detected in all tumor-bearing mice, rapidly declining after B-RAF inhibitor treatment. CTCs were shed early from localized tumors and a short course of B-RAF inhibition following surgical resection was sufficient to dramatically suppress distant metastases. The large number of CTCs in melanoma-bearing mice enabled comparison of RNA sequencing profiles with the matched primary tumor. A mouse melanoma CTC-derived signature correlated with invasiveness and cellular motility in human melanoma. In patients with metastatic melanoma, CTCs were detected in smaller numbers in patients with metastatic melanoma and declined with successful B-RAF targeted therapy. Together, the capture of CTCs and their molecular characterization provide insight into the hematogenous spread of melanoma. We adapted a microfluidic platform, the HbCTC-Chip (Stott et al., 2010, Pubmed ID: 20930119), to capture melanoma CTCs derived from mouse tumors, using panels of antibodies against melanoma-specific cell surface markers, followed by staining for melanoma antigens and optimized on-chip fluorescent imaging. We used a tamoxifen inducible BRAF(CA/+)/PTEN(flox/flox) melanoma mouse model (Dankort et al., 2009, Pubmed ID: 19282848) derived from a C57BL/6 background. Such mice received focal subcutaneous injection of tamoxifen (Sigma) (50ul at 5mg/ml in 50% EtOH suspension) at the left flank at 6-7 weeks after birth. Blood samples were collected from five mice with high tumor burden following tamoxifen injection. Blood from each mouse was split and processed through the CTC-chips functionalized with anti-CSPG4/MCAM antibody and control IgGs, respectively. Matched primary (from the tamoxifen injection site) and metastatic (from upper or lower back) tumors were harvested from the same mouse and immediately flash-frozen in liquid nitrogen. RNA extraction, single molecular sequencing and determination of Digital Gene Expression was as in Yu et al., 2012 (Pubmed ID: 22763454). One of the five mice yielded no CTC or IgG data. In addition, skin was taken from a sixth mouse, which was a complete wild type C57BL/6 mouse (without the BRAF/PTEN transgenes).The skin was taken after euthanasia of the animal and was processed as the tissue from the matched primary and metastatic tumors.

Dataset Information

LC-MS/MS data for HCA mRNA antibodies

Dataset's files

Publications

Platform-Specific Fc <i>N</i>-Glycan Profiles of an Antisperm Antibody.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

LC-MS/MS data for HCA mRNA antibodies

Dataset's files

Publications

Platform-Specific Fc &lt;i&gt;N&lt;/i&gt;-Glycan Profiles of an Antisperm Antibody.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Platform-Specific Fc <i>N</i>-Glycan Profiles of an Antisperm Antibody.