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Pleiotropic effects of PhaR regulator in Bradyrhizobium diazoefficiens microaerobic metabolism

ABSTRACT: Identification of PHB Granule-associated Proteins in B. diazoefficiens and a phaR mutant, both cultured under microoxic conditions (0.5% O2) in Götz minimal medium supplemented with mannitol.

INSTRUMENT(S): amaZon Speed ETD

ORGANISM(S): Bacteria Bradyrhizobium Japonicum Usda 110

SUBMITTER: Juan Ignacio Quelas

LAB HEAD: Juan Ignacio Quelas

PROVIDER: PXD047962 | Pride | 2024-05-21

REPOSITORIES: Pride

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			Action	DRS
	1_5055.dat	Other
	1_5055.mzid.gz	Mzid
	1_5055.zip	Other
	1_5057.dat	Other
	1_5057.mzid.gz	Mzid

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Publications

Pleiotropic Effects of PhaR Regulator in Bradyrhizobium diazoefficiens Microaerobic Metabolism.

Quelas Juan I JI Cabrera Juan J JJ Díaz-Peña Rocío R Sánchez-Schneider Lucía L Jiménez-Leiva Andrea A Tortosa Germán G Delgado María J MJ Pettinari M Julia MJ Lodeiro Aníbal R AR Del Val Coral C Mesa Socorro S

International journal of molecular sciences 20240210 4

Bradyrhizobium diazoefficiens can live inside soybean root nodules and in free-living conditions. In both states, when oxygen levels decrease, cells adjust their protein pools by gene transcription modulation. PhaR is a transcription factor involved in polyhydroxyalkanoate (PHA) metabolism but also plays a role in the microaerobic network of this bacterium. To deeply uncover the function of PhaR, we applied a multipronged approach, including the expression profile of a phaR mutant ...[more]

PMID: 38396833

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Pleiotropic effects of PhaR regulator in Bradyrhizobium diazoefficiens microaerobic metabolism

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Pleiotropic effects of PhaR regulator in Bradyrhizobium diazoefficiens microaerobic metabolism

Project description:Bradyrhizobium diazoefficiens can live inside soybean root nodules and in free-living conditions. In both states, when oxygen levels decrease, cells adjust their protein pools by gene transcription modulation. PhaR encodes a transcription factor annotated as PHA (polyhydroxyalkanoate) accumulation regulator. We found that PhaR not only controls the PHA cycle but also acts as a global regulator of excess carbon allocation by controlling the expression of fixK2 and nifA genes, both encoding key transcription factors for microoxic and symbiotic metabolism in B. diazoefficiens. The function of PhaR was expanded by a multi-pronged approach that includes analysis of the effects of phaR mutation at transcriptional and protein levels of putative PhaR targets and direct control mediated by PhaR determined by EMSA assays. We also were able to identify PhaR, phasins and other proteins associated with PHA granules which confirmed a global function of PhaR in microoxia.

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