A genome-wide screen identifies silencers with distinct chromatin properties and mechanisms of repression

ABSTRACT: Differential gene transcription enables development and homeostasis in all animals and is regulated by two major classes of distal cis-regulatory DNA elements (CREs), enhancers and silencers. While enhancers have been thoroughly characterized, the properties and mechansisms of silencers remain largely unknown. By an unbiased genome-wide functional screen in Drosophila melanogaster S2 cells, we discover a class of silencers that bind one of three transcription factors (TFs) and are generally not included in chromatin-defined CRE catalogs, as they mostly lack detectable DNA accessibility. The silencer-binding TF CG11247, which we term Saft, safeguards cell fate decisions in vivo and functions via a highly-conserved domain we term ZAC and the corepressor G9a, independently of G9a’s H3K9-methyltransferase activity. Overall, our identification of silencers with unexpected properties and mechanisms has important implications for the understanding and future study of repressive CREs, as well as the functional annotation of animal genomes.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Schneider-2 Cell

SUBMITTER: Richard Imre  

LAB HEAD: Alexander Stark

PROVIDER: PXD047987 | Pride | 2024-11-13

REPOSITORIES: pride