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Molecular-Level Architecture of Chlamydomonas reinhardtii’s Glycoprotein-Rich Cell Wall

ABSTRACT: Microalgae are a renewable and promising biomass for large-20 scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level model of the non-crystalline and water-insoluble glycoprotein-rich cell wall of Chlamydomonas reinhardtii. Using in situ solid-state and sensitivity-enhanced nuclear magnetic 25 resonance, we reveal unprecedented details on the protein and carbohydrate composition and their nanoscale heterogeneity, as well as the presence of spatially segregated protein- and glycan-rich regions with different dynamics and hydration levels. We show that mannose-rich lower molecular-weight proteins likely contribute to the cell wall cohesion by binding to high-molecular weight protein components, and that water provides plasticity to the cell-wall architecture. The 30 structural insight exemplifies strategies used by nature to form cell walls devoid of cellulose or other glycan polymers.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Adnan Halim

LAB HEAD: Adnan Halim

PROVIDER: PXD048024 | Pride | 2024-01-09

REPOSITORIES: Pride

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			Action	DRS
	230511_Fusion_NewObj_AH_Chlaydomonas_CW_Tryp_LWAC_E1.raw	Raw
	230511_Fusion_NewObj_AH_Chlaydomonas_CW_Tryp_LWAC_E1_6.msf	Msf
	230511_Fusion_NewObj_AH_Chlaydomonas_CW_Tryp_LWAC_E2.raw	Raw
	230511_Fusion_NewObj_AH_Chlaydomonas_CW_Tryp_LWAC_E3.raw	Raw
	230511_Fusion_NewObj_AH_Chlaydomonas_CW_Tryp_LWAC_E4.raw	Raw

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