Proteomics

Dataset Information

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SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23


ABSTRACT: Here we characterized SRSF1’s interactome using proximity labeling and mass spectrometry. This approach yielded 190 proteins enriched in the SRSF1 samples, independently of the N- or C-terminal location of the biotin-labeling domain. The detected proteins reflect established functions of SRSF1 in pre-mRNA splicing and reveal additional connections to spliceosome proteins, in addition to other recently identified functions. We validated a robust interaction with the spliceosomal RNA helicase DDX23/PRP28 using bimolecular fluorescence complementation and in vitro binding assays. The interaction is mediated by the N-terminal RS-like domain of DDX23 and both RRM1 and the RS domain of SRSF1.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Paolo Cifani  

LAB HEAD: Paolo Cifani

PROVIDER: PXD048180 | Pride | 2024-04-30

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
220309_DS_BS1.raw Raw
220309_DS_BS2.raw Raw
220309_DS_BS3.raw Raw
220309_DS_G1.raw Raw
220309_DS_G2.raw Raw
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