Dataset Information

The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling

ABSTRACT: Plasma proteins are considered to be the most informative source of biomarkers for disease diagnosis and monitoring. Mass spectrometry (MS)-based proteomics has been applied to discover biomarkers in plasma, but the complexity of plasma proteins and the extremely large dynamic range of protein abundances make the application of plasma proteomics in the clinic a great challenge. We have designed and synthesized zeolite-based nanoparticles for the depletion of high-abundant plasma proteins in plasma. This novel plasma proteomic assay is capable of consistently detecting around 3000 plasma proteins in a 45-minute gradient. Compared to neat and depleted plasma, the plasma proteins identified by our assay show distinct biological profile, which is validated in several public datasets. A pilot investigation of the proteomic profile of hepatocellular carcinoma (HCC) cohort identified 15 promising protein features, highlighting the diagnostic value of plasma proteome in distinguishing HCC from healthy individuals. Furthermore, this assay can be easily integrated with all current downstream protein profiling methods and potentially extended to other biofluids. In conclusion, we have established a robust and efficient plasma proteomic assay with unprecedented identification depths, paving the way for the translation of plasma proteomics into clinical applications.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Jingnan Huang

LAB HEAD: Jian Guan

PROVIDER: PXD048186 | Pride | 2024-05-23

REPOSITORIES: Pride

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			Action	DRS
	20221218_HJN_human_plasma_liver_cancer_122.raw	Raw
	20221218_HJN_human_plasma_liver_cancer_122.raw.quant	Raw
	20221218_HJN_human_plasma_liver_cancer_135.raw	Raw
	20221218_HJN_human_plasma_liver_cancer_135.raw.quant	Raw
	20221218_HJN_human_plasma_liver_cancer_136.raw	Raw

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The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling.

Li Nan N Huang Jingnan J He Shangwen S Zheng Qiaocong Q Ye Feng F Qin Zhengxing Z Wang Dong D Xiao Ting T Mao Mengyuan M Zhou Zhenhua Z Tang Tingxi T Zhang Longshan L Wang Xiaoqing X Wang Yingqiao Y Lyu Ying Y Liu Laiyu L Dai Lingyun L Wang Jigang J Guan Jian J

Journal of nanobiotechnology 20240410 1

Plasma proteins are considered the most informative source of biomarkers for disease diagnosis and monitoring. Mass spectrometry (MS)-based proteomics has been applied to identify biomarkers in plasma, but the complexity of the plasma proteome and the extremely large dynamic range of protein abundances in plasma make the clinical application of plasma proteomics highly challenging. We designed and synthesized zeolite-based nanoparticles to deplete high-abundance plasma proteins. The resulting no ...[more]

PMID: 38600601

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