Proteomics

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IGF1R signaling primes epithelial cancer cells to induce epithelial-mesenchymal plasticity by upregulating integrin αV in cutaneous squamous cell carcinoma

ABSTRACT: Asses the global (phospho) protein profile of cancer cell lysates of three cell biological replicates of each stage of our model of skin SCC progression (full epithelial, plastic EpCAM-positive, plastic EpCAM-negative, and full mesenchymal) isolated from mouse skin SCCs by FACS sorting. The main research question in this experiment using pTyrIP, total protein lysate, and TiOx profiling is to check for differences in full epithelial vs plastic EpCAM(+) cancer cells, and plastic EpCAM(+) vs plastic EpCAM(-) cancer cells. With this information we would like to identify kinase signalling pathways that are induced in each of these stages and that may promote SCC progression, in order to design strategies to block tumor progression.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Thang Pham  

LAB HEAD: Connie Jimenez

PROVIDER: PXD048323 | Pride | 2024-08-10

REPOSITORIES: Pride

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IGF1R signaling induces epithelial-mesenchymal plasticity via ITGAV in cutaneous carcinoma.

Lopez-Cerda Marta M   Lorenzo-Sanz Laura L   da Silva-Diz Victoria V   Llop Sandra S   Penin Rosa M RM   Bermejo Josep Oriol JO   de Goeij-de Haas Richard R   Piersma Sander R SR   Pham Thang V TV   Jimenez Connie R CR   Martin-Liberal Juan J   Muñoz Purificación P  

Journal of experimental & clinical cancer research : CR 20240729 1

<h4>Background</h4>Early cutaneous squamous cell carcinomas (cSCCs) generally show epithelial differentiation features and good prognosis, whereas advanced cSCCs present mesenchymal traits associated with tumor relapse, metastasis, and poor survival. Currently, the mechanisms involved in cSCC progression are unclear, and the established markers are suboptimal for accurately predicting the clinical course of the disease.<h4>Methods</h4>Using a mouse model of cSCC progression, expression microarra  ...[more]

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