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Activity guided profiling of yeast proteasome - protein extraction test

ABSTRACT: This experiment compared the impact of protein extraction conditions on the identification and quantification of proteasome subunits in WT yeast. It involved comparing proteins extracted from identical WT yeast samples under two different conditions: native and denaturing (in the presence of SDS and TCA).

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Oded Kleifeld

LAB HEAD: Oded Kleifeld

PROVIDER: PXD048380 | Pride | 2024-02-15

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Seq83144_88min_480Ex1.raw	Raw
	Seq83145_88min_480Ex1.raw	Raw
	Seq83148_88min_480Ex1.raw	Raw
	Seq83149_88min_480Ex1.raw	Raw
	Yeast_Jan2020.fasta	Fasta

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Publications

Activity-Guided Proteomic Profiling of Proteasomes Uncovers a Variety of Active (and Inactive) Proteasome Species.

Sahoo Manisha Priyadarsini MP Lavy Tali T Cohen Noam N Sahu Indrajit I Kleifeld Oded O

Molecular & cellular proteomics : MCP 20240129 3

Proteasomes are multisubunit, multicatalytic protein complexes present in eukaryotic cells that degrade misfolded, damaged, or unstructured proteins. In this study, we used an activity-guided proteomic methodology based on a fluorogenic peptide substrate to characterize the composition of proteasome complexes in WT yeast and the changes these complexes undergo upon the deletion of Pre9 (Δα3) or of Sem1 (ΔSem1). A comparison of whole-cell proteomic analysis to activity-guided proteasome profiling ...[more]

PMID: 38296025

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