Dataset Information

Deciphering the role of PGRMC2 in the human endometrium during the menstrual cycle and in vitro decidualization using an in vitro approach

ABSTRACT: Understanding how P4 signaling occurs in endometrial cells is important to guide the development of more effective therapeutic approaches to the numerous reproductive diseases caused by disrupted P4 signaling, resulting in subfertility or infertility (Bunch et al., 2013; Kim et al., 2013; Zhang and Murphy, 2014; Szekeres-Bartho, 2018; Hirota, 2019; Liao et al., 2019). Here, to fill these knowledge gaps, we characterized endometrial PGRMC2 expression throughout the human menstrual cycle and in hEnSCs subjected to an in vitro model of human endometrial decidualization. Additionally, we describe the functional implications of PGRMC2 with a silencing approach assay, integrating RNA-seq and proteomic techniques. We evaluated the functional implication of PGRMC2 in the embryo implantation process with an outgrowth in vitro model. Finally, we also compared endometrial PGRMC2 expression with PGRMC1 and PGR.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endometrium Epithelium

DISEASE(S): Female Infertility Of Uterine Origin

SUBMITTER: Luz Valero

LAB HEAD: Francisco Dominguez

PROVIDER: PXD048494 | Pride | 2024-06-22

REPOSITORIES: Pride

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			Action	DRS
	FD_AQ_221019_20_25_LIB_SP_FDR__FDR.xlsx	Xlsx
	FD_AQ_221025OK_L1_Zt.wiff	Wiff
	FD_AQ_221025OK_L1_Zt.wiff.scan	Wiff
	FD_AQ_221025_L2_Zt.wiff	Wiff
	FD_AQ_221025_L2_Zt.wiff.scan	Wiff

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Deciphering the role of PGRMC2 in the human endometrium during the menstrual cycle and in vitro decidualization using an in vitro approach.

Medina-Laver Yassmin Y Gonzalez-Martin Roberto R de Castro Pedro P Diaz-Hernandez Indra I Alama Pilar P Quiñonero Alicia A Palomar Andrea A Dominguez Francisco F

Human reproduction (Oxford, England) 20240501 5

<h4>Study question</h4>What is the human endometrial non-classical progesterone receptor (PGR) membrane component 2 (PGRMC2) expression pattern throughout the menstrual cycle and what role does it play during decidualization?<h4>Summary answer</h4>Endometrial PGRMC2 expression fluctuates during the human menstrual cycle and is abundantly expressed in human endometrial stromal cells (hEnSCs) during in vitro decidualization, process where PGRMC2 is involved in embryo implantation-related pathways. ...[more]

PMID: 38452349

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