Dataset Information

Comparative analysis of retroviral Gag-host cell interactions in the nucleus

ABSTRACT: Retroviruses exploit a variety of host proteins to assemble and release virions from infected cells. To date, most studies that examined possible interacting partners of retroviral Gag proteins focused on host proteins that localize primarily to the cytoplasm or plasma membrane. Given the recent findings that several full-length Gag proteins localize to the nucleus, identifying the Gag-nuclear interactome has high potential for novel findings that reveal previously unknown host processes. This dataset contains results from a mass spectrometry approach using affinity-tagged (His6) HIV-1 and RSV Gag proteins mixed with nuclear extracts, and identifies potential binding partners of HIV-1 and RSV Gag involved in several nuclear processes, including transcription, splicing, RNA modification, and chromatin structure. Although a subset of host proteins interacted with both Gag proteins, there were also unique host proteins belonging to each interactome dataset. These results provide a strong premise for future functional studies to investigate roles for these nuclear host factors that may have shared functions in the biology of both retroviruses, as well as functions specific to RSV and HIV-1, given their distinctive hosts and molecular pathology.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human) Gallus Gallus (chicken)

TISSUE(S): Cell Culture

SUBMITTER: Leslie Parent

LAB HEAD: Leslie Joan Parent

PROVIDER: PXD048774 | Pride | 2024-07-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Beads_DF1_Dataset_1.group	Other
	Beads_DF1_Dataset_1.wiff	Wiff
	Beads_DF1_Dataset_1.wiff.scan	Wiff
	Beads_DF1_Dataset_2.group	Other
	Beads_DF1_Dataset_2.wiff	Wiff

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Publications

Comparative analysis of retroviral Gag-host cell interactions: focus on the nuclear interactome.

Lambert Gregory S GS Rice Breanna L BL Maldonado Rebecca J Kaddis RJK Chang Jordan J Parent Leslie J LJ

Retrovirology 20240619 1

Retroviruses exploit host proteins to assemble and release virions from infected cells. Previously, most studies focused on interacting partners of retroviral Gag proteins that localize to the cytoplasm or plasma membrane. Given that several full-length Gag proteins have been found in the nucleus, identifying the Gag-nuclear interactome has high potential for novel findings involving previously unknown host processes. Here we systematically compared nuclear factors identified in published HIV-1 ...[more]

PMID: 38898526

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