Dataset Information

Observations from the proteomics bench - Quality Assessment / Experiment Reproducibility - QExHF2019

ABSTRACT: These project contains 10 technical replicate measurements of a standard sample, that is used to assess the quality control and instrument performance in between real project samples. It was used in a viewpoint paper to illustrate the challenges. The samples were measured with three different machines, one is given in this project, two other projects for the other machines exist.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell Of Lung, Alveolar Epithelial Cell Line

DISEASE(S): Lung Adenocarcinoma

SUBMITTER: Karin Schork

LAB HEAD: Martin Eisenacher

PROVIDER: PXD048839 | Pride | 2024-02-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20230223_human_proteome_with_spikeins.fasta	Fasta
	QExHF10717std.mzML	Mzml
	QExHF10717std.mzTab.gz	Mztab
	QExHF10717std.raw	Raw
	QExHF10717std.txt	Txt

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Publications

Observations from the Proteomics Bench.

König Simone S Schork Karin K Eisenacher Martin M

Proteomes 20240206 1

Many challenges in proteomics result from the high-throughput nature of the experiments. This paper first presents pre-analytical problems, which still occur, although the call for standardization in omics has been ongoing for many years. This article also discusses aspects that affect bioinformatic analysis based on three sets of reference data measured with different orbitrap instruments. Despite continuous advances in mass spectrometer technology as well as analysis software, data-set-wise qu ...[more]

PMID: 38390966

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