Proteomics

Dataset Information

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Auto-sumoylation of the Ubc9 E2 SUMO-conjugating Enzyme Extends Cellular Lifespan


ABSTRACT: Calorie restriction (CR) provides anti-aging benefits through diverse processes, such as reduced metabolism and growth and increased mitochondrial activity. Although controversy still exists regarding CR-mediated lifespan effects, many researchers are seeking interventions that mimic the effects of CR. Yeast has proven to be a useful model system for aging studies, including CR effects. We report here that yeast adapted through in vitro evolution to the severe cellular stress caused by loss of the Ulp2 SUMO-specific protease exhibit both enhanced growth rates and replicative lifespan, and they have altered gene expression profiles similar to those observed in CR. Notably, in certain evolved ulp2Δ lines, a dramatic increase in the auto-sumoylation of Ubc9 E2 SUMO-conjugating enzyme results in altered regulation of multiple targets involved in energy metabolism and translation at both transcriptional and post-translational levels. This increase is essential for the survival of aged cells and CR-mediated lifespan extension. Thus, we suggest that high Ubc9 auto-sumoylation exerts potent anti-aging effects by promoting efficient energy metabolism-driven improvements in cell replication abilities. This potential could be therapeutically explored for the development of a novel CR mimetic strategy.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) Saccharomyces Cerevisiae

SUBMITTER: Dong Won Jeong  

LAB HEAD: Hong Yeoul, Ryu

PROVIDER: PXD048944 | Pride | 2025-01-15

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
221214_JEJ_KBU_2kr.raw Raw
221214_JEJ_KBU_Notag.raw Raw
221214_JEJ_KBU_WT.raw Raw
221215_JEJ_KBU_Comparison.pdResult Other
checksum.txt Txt
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Publications

Auto-sumoylation of the Ubc9 E2 SUMO-conjugating Enzyme Extends Cellular Lifespan.

Ryu Hong-Yeoul HY   Jeong Dong-Won DW   Kim Seung Yeon SY   Jeoung Seok-Won SW   Zhao Dejian D   Knight James J   Lam TuKiet T   Jin Jong Hwa JH   Lee Hyun-Shik HS   Hochstrasser Mark M  

Research square 20240321


Calorie restriction (CR) provides anti-aging benefits through diverse processes, such as reduced metabolism and growth and increased mitochondrial activity. Although controversy still exists regarding CR-mediated lifespan effects, many researchers are seeking interventions that mimic the effects of CR. Yeast has proven to be a useful model system for aging studies, including CR effects. We report here that yeast adapted through <i>in vitro</i> evolution to the severe cellular stress caused by lo  ...[more]

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