Dataset Information

The proteome of Tumor-associated macrophages in laryngeal squamous cell carcinoma

ABSTRACT: Differential protein expression in LSCC with negative lymphatic metastasis (LNM-) and LSCC with positive lymphatic metastasis (LNM+) TAMs was subsequently analyzed by 4D label-free proteomics.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Larynx, Macrophage

DISEASE(S): Laryngeal Squamous Cell Carcinoma

SUBMITTER: Licheng Xu

LAB HEAD: Linli Tian

PROVIDER: PXD048960 | Pride | 2024-02-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	QD055SCB1_C1_Slot2-39_1_16332.d.zip	Other
	QD055SCB1_C2_Slot2-40_1_16333.d.zip	Other
	QD055SCB1_C3_Slot2-41_1_16334.d.zip	Other
	QD055SCB1_LSCC1_Slot2-36_1_16328.d.zip	Other
	QD055SCB1_LSCC2_Slot2-37_1_16329.d.zip	Other

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Project description:Acute compartment syndrome (ACS) is a disease in which local circulation is affected due to increased pressure within the compartment. We previously found in patients with calf fractures, the pressure of fascial compartment could be sharply reduced upon the appearance of tension blisters. Deep fascia, as the important structure for compartment, might play key role in this process. Therefore, the aim of the present study was to examine the differences in gene profile in deep fascia tissue in fracture patients of the calf with or without tension blisters, and to explore the role of fascia in pressure improvement in ACS. Patients with lower leg fracture were enrolled and divided into without tension blister control group (CON group, n=10), and with tension blister group (TB group, n=10). Deep fascia tissues were collected and LC-MS/MS label-free quantitative proteomics were performed. Genes involved in fascia structure and fibroblast function were further validated by Western blot. The differentially expressed proteins were found to be mainly enriched in pathways related to protein synthesis and processing, stress fiber assembly, cell-substrate adhesion, leukocyte mediated cytotoxicity, and cellular response to stress. Compared with the CON group, the expression of Peroxidasin homolog (PXDN), which promotes the function of fibroblasts, and Leukocyte differentiation antigen 74 (CD74), which enhances the proliferation of fibroblasts, were significantly upregulated, while the expression of Matrix metalloproteinase-9 (MMP9), which is involved in collagen hydrolysis, and Neutrophil elastase (ELANE), which is involved in elastin hydrolysis, were significantly reduced in the TB group (p all <0.05), indicating fascia tissue underwent microenvironment reconstruction during ACS.In summary, the ACS accompanied by blisters is associated with the enhanced function and proliferation of fibroblasts and reduced hydrolysis of collagen and elastin. The adaptive alterations in the stiffness and elasticity of the deep fascia might be crucial for pressure release of ACS.

Dataset Information

The proteome of Tumor-associated macrophages in laryngeal squamous cell carcinoma

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