Dataset Information

TMT-based phosphoproteomics and proteomics analysis of the mouse sperm

ABSTRACT: In this study, we conducted a comprehensive quantitative phosphoproteomic analysis of mouse epididymal sperm from different regions (caput, corpus, and cauda) to unveil the dynamics of protein phosphorylation during sperm maturation. And we performed phosphoproteomic analysis of ALK inhibitor 2 compound 18-treated and control sperm to gain further insights into the potential mechanisms by which TSSK2 regulates sperm motility.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spermatozoid

SUBMITTER: xiangzheng zhang

LAB HEAD: xuejiang guo

PROVIDER: PXD049067 | Pride | 2024-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MaxQuant_v2.2.0.0.zip	Other
	TMT15_epididymal_sperm.zip	Other
	TMT15_epididymal_sperm_HPRP_F10_20230214.raw	Raw
	TMT15_epididymal_sperm_HPRP_F11_20230215.raw	Raw
	TMT15_epididymal_sperm_HPRP_F12_20230215.raw	Raw

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Publications

Quantitative Phosphoproteomic Profiling of Mouse Sperm Maturation in Epididymis Revealed Kinases Important for Sperm Motility.

Zhang Xiangzheng X Tu Haixia H Zhou Xin X Wang Bing B Guo Yueshuai Y Situ Chenghao C Qi Yaling Y Li Yan Y Guo Xuejiang X

Molecular & cellular proteomics : MCP 20240706 8

Transcriptionally and translationally silent sperm undergo functional maturation during epididymis traverse, which provides sperm ability to move and is crucial for successful fertilization. However, the molecular mechanisms governing sperm maturation remain poorly understood, especially at the protein post-translational modification level. In this study, we conducted a comprehensive quantitative phosphoproteomic analysis of mouse epididymal sperm from different regions (caput, corpus, and cauda ...[more]

PMID: 38977202

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