Proteomics

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Quantitative Proteomics Reveals Cellular Responses to Stress and Tunicamycin in CHO Cells

ABSTRACT: We conducted proteomics analysis aimed at evaluating the differences between CHO cell lines expressing 2G12 and 353/11, cells were cultured using a semi-perfusion batch process in parallel. The cells were collected and pelleted on day six, four hours after medium exchange, when both cell lines had reached maximum density and exhibited sustained viability (Figure 1). One cell line exhibited high production (353/11), whereas the other one (2G12) displayed low production. To induce endoplasmic reticulum (ER) stress, 353/11 cells were cultivated separately and parallelly treated with tunicamycin (TM) at a final concentration of 1 µg/mL in fresh cultivation medium for four hours prior to harvesting. This approach aimed to establish uniform experimental conditions comparable to those of non-treated cells.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cho-k1 Cell

SUBMITTER: Eldi Sulaj  

LAB HEAD: Gorji Marzban

PROVIDER: PXD049297 | Pride | 2024-07-03

REPOSITORIES: Pride

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Quantitative proteomics reveals cellular responses to individual mAb expression and tunicamycin in CHO cells.

Sulaj Eldi E   Schwaigerlehner Linda L   Sandell Felix L FL   Dohm Juliane C JC   Marzban Gorji G   Kunert Renate R  

Applied microbiology and biotechnology 20240619 1

Chinese hamster ovary (CHO) cells are popular in the pharmaceutical industry for their ability to produce high concentrations of antibodies and their resemblance to human cells in terms of protein glycosylation patterns. Current data indicate the relevance of CHO cells in the biopharmaceutical industry, with a high number of product commendations and a significant market share for monoclonal antibodies. To enhance the production capabilities of CHO cells, a deep understanding of their cellular a  ...[more]

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