Proteomics

Dataset Information

0

Integrative single-plaque analysis reveals signature Aβ and lipid profiles in the Alzheimer’s brain


ABSTRACT: Utilizing multimodal mass spectrometry imaging (MSI) combined with machine learning techniques, this study investigates the molecular heterogeneity of amyloid-β (Aβ) plaques and associated lipid profiles in post-mortem brain samples from Alzheimer’s disease (AD) and amyloid-positive cognitively unaffected (AP-CU) individuals. Our analytical approach permitted single-plaque level investigation, revealing distinct populations of amyloid plaques characterized by differential Aβ and lipid compositions. Notably, the integration of MSI data with machine learning based feature extraction enabled the identification of Aβ38 and ganglioside GM1 as significant molecular markers differentiating AD from AP-CU pathology. These findings suggest that the heterogeneity in Aβ metabolism and lipid homeostasis, as revealed through precise analysis, is a key factor in the pathogenesis of AD and implies that total amyloid burden alone is an insufficient marker for the disease. The application of MSI and machine learning based feature extraction in this context exemplifies a progressive analytic strategy to unravel complex biochemical phenomena, offering potential pathways for the refinement of diagnostic tools and deepening the understanding of neurodegenerative diseases from an analytical chemistry perspective.

INSTRUMENT(S): rapifleX, timsTOF fleX

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

DISEASE(S): Alzheimer's Disease

SUBMITTER: Thomas Enzlein  

LAB HEAD: Carsten Hopf

PROVIDER: PXD049325 | Pride | 2024-06-06

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AP-CU_pride.txt Txt
S15_210716_pa_ucl_pa_neg.ibd Other
S15_210716_pa_ucl_pa_neg.imzML Other
S15_210716_pa_ucl_s15_pa_pep.ibd Other
S15_210716_pa_ucl_s15_pa_pep.imzML Other
Items per page:
1 - 5 of 66

Similar Datasets

2021-11-15 | PXD028949 | Pride
2020-10-15 | PXD020824 | Pride
2022-02-15 | PXD019653 | Pride
2022-02-15 | PXD019662 | Pride
2022-02-15 | PXD019666 | Pride
2022-02-17 | PXD025594 | Pride
2024-01-23 | PXD037038 | Pride
2020-08-06 | PXD011104 | Pride
2021-09-09 | PXD023196 | Pride
2021-03-25 | PXD014274 | Pride