Proteomics

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SRF is a new player in 3D genome organization and stem cell pluripotency


ABSTRACT: Serum response factor (SRF) is a transcription factor essential for cell proliferation, differentiation, and migration, and is required for primitive streak and mesoderm formation in the embryo. The canonical roles of SRF are mediated by a diverse set of context-dependent cofactors. Here we show that SRF physically interacts with CTCF and cohesin subunits at TAD boundaries and loop anchors. SRF reinforces the insulation of TADs and promotes the formation of long-range chromatin loops. In ES cells, SRF associates with Oct4, Sox2, and Nanog and contributes to the formation of 3D pluripotency hubs. Our findings reveal new roles of SRF in higher-order chromatin organization.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: David Meierhofer  

LAB HEAD: Bernhard Herrmann

PROVIDER: PXD049365 | Pride | 2024-10-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CAP_1.raw Raw
CAP_2.raw Raw
CAP_3.raw Raw
CAP_4.raw Raw
CMA_1.raw Raw
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