Proteomics

Dataset Information

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The S4-domain containing protein YlmH is involved in ribosome-associated quality control in Bacillus subtilis

ABSTRACT: By taking advantage of the strong genetic interactions between trans-translation and other ribosome rescue systems, we have employed a transposon sequencing (Tn-Seq) to identify potential novel rescue factors in Bacillus subtilis. In addition to the identification the ArfA-type rescue factor BrfA, as well as the RQC elongation actors RqcP and RqcH, our Tn-Seq screen led to the identification of YlmH, a poorly characterized S4-domain-containing protein, as a potential RQC factor. Binding of YlmH to 50S ribosomal subunit was confirmed by proteomics approach.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

SUBMITTER: Tanel Tenson  

LAB HEAD: Vasili Hauryliuk

PROVIDER: PXD049370 | Pride | 2024-09-16

REPOSITORIES: Pride

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201111_03_Orbi1_KN_SER_G90_T115_Paternoga_control_184.raw Raw
201111_05_Orbi1_KN_SER_G90_T115_Paternoga_control_185.raw Raw
201111_07_Orbi1_KN_SER_G90_T115_Paternoga_yabN_184.raw Raw
201111_09_Orbi1_KN_SER_G90_T115_Paternoga_yabR_184.raw Raw
201111_11_Orbi1_KN_SER_G90_T115_Paternoga_yacO_185.raw Raw
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Publications

Ribosomes trapped on mRNAs during protein synthesis need to be rescued for the cell to survive. The most ubiquitous bacterial ribosome rescue pathway is trans-translation mediated by tmRNA and SmpB. Genetic inactivation of trans-translation can be lethal, unless ribosomes are rescued by ArfA or ArfB alternative rescue factors or the ribosome-associated quality control (RQC) system, which in Bacillus subtilis involves MutS2, RqcH, RqcP and Pth. Using transposon sequencing in a trans-translation-i  ...[more]

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