Proteomics

Dataset Information

0

Differentiating children with sepsis with and without acute respiratory distress syndrome using proteomics


ABSTRACT: Both sepsis and acute respiratory distress syndrome (ARDS) rely on imprecise clinical definitions leading to heterogeneity, which has contributed to negative trials. Because circulating protein/DNA complexes have been implicated in sepsis and ARDS, we aimed to develop a proteomic signature of DNA-bound proteins to discriminate between children with sepsis with and without ARDS. We performed a prospective case-control study in 12 children with sepsis with ARDS matched to 12 children with sepsis without ARDS on age, severity of illness score, and source of infection. We performed co-immunoprecipitation and downstream proteomics in plasma collected ≤ 24 h of intensive care unit admission. Expression profiles were generated, and a random forest classifier was used on differentially expressed proteins to develop a signature which discriminated ARDS. The classifier was tested in six independent blinded samples. Neutrophil and nucleosome proteins were over-represented in ARDS, including two S100A proteins, superoxide dismutase (SOD), and three histones. Random forest produced a 10-protein signature that accurately discriminated between children with sepsis with and without ARDS. This classifier perfectly assigned six independent blinded samples as having ARDS or not. We validated higher expression of the most informative discriminating protein, galectin-3-binding protein, in children with ARDS. Our methodology has applicability to isolation of DNA-bound proteins from plasma. Our results support the premise of a molecular definition of ARDS, and give preliminary insight into why some children with sepsis, but not others, develop ARDS.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hossein Fazelinia  

LAB HEAD: Nadir Yehya, MD

PROVIDER: PXD049377 | Pride | 2024-02-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20190301_162404_NY1313_1326_1351.sne Other
NY1313_1326_1351_Peptide_Report.csv Csv
NY1313_1326_1351_Protein_Report.csv Csv
Spectral_Library_DDA.zip Other
checksum.txt Txt
Items per page:
1 - 5 of 23
altmetric image

Publications

Differentiating children with sepsis with and without acute respiratory distress syndrome using proteomics.

Yehya Nadir N   Fazelinia Hossein H   Taylor Deanne M DM   Lawrence Gladys G GG   Spruce Lynn A LA   Thompson Jill M JM   Margulies Susan S SS   Seeholzer Steven H SH   Worthen G Scott GS  

American journal of physiology. Lung cellular and molecular physiology 20220105 3


Both sepsis and acute respiratory distress syndrome (ARDS) rely on imprecise clinical definitions leading to heterogeneity, which has contributed to negative trials. Because circulating protein/DNA complexes have been implicated in sepsis and ARDS, we aimed to develop a proteomic signature of DNA-bound proteins to discriminate between children with sepsis with and without ARDS. We performed a prospective case-control study in 12 children with sepsis with ARDS matched to 12 children with sepsis w  ...[more]

Similar Datasets

2015-04-01 | E-GEOD-66890 | biostudies-arrayexpress
2012-07-10 | E-GEOD-32707 | biostudies-arrayexpress
2023-09-01 | GSE242127 | GEO
2015-04-01 | GSE66890 | GEO
2012-07-11 | GSE32707 | GEO
2020-07-10 | GSE151263 | GEO
2018-12-31 | GSE84439 | GEO
2022-04-06 | PXD025125 | Pride
2021-05-16 | GSE150707 | GEO
2009-01-05 | E-GEOD-13904 | biostudies-arrayexpress