Dataset Information

Effect of epinephrine on a proteome of Cutibacterium acnes

ABSTRACT: The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies. In the present investigation, we studied the proteome of biofilms and planktonic cultures of skin actinobacterium Cutibacterium acnes. In our investigation, we tested the effect of epinephrine in concentrations 5 nM (normal blood level) and 5 µM (1000-fold higher) on 72 h biofilms and planktonic cultures in comparison with controls without the hormone administration. Also, we compared the samples treated with epinephrine in different concentrations. Also, we investigated the differences between planktonic cultures and biofilms and how the hormone influences those differences. The present database contains the raw data of proteins changes in their concentrations in described conditions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Cutibacterium Acnes Subsp. Defendens Atcc 11828

TISSUE(S): Cell Culture

SUBMITTER: Rustam Ziganshin

LAB HEAD: Yury A. Nikolayev

PROVIDER: PXD049410 | Pride | 2024-07-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Description_of_samples.zip	Other
	GAN_231213_004_RZ_GAN-1.raw	Raw
	GAN_231213_005_RZ_GAN-2.raw	Raw
	GAN_231213_006_RZ_GAN-3.raw	Raw
	GAN_231213_008_RZ_GAN-4.raw	Raw

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Publications

Proteomic and transcriptomic analyses of <i>Cutibacterium acnes</i> biofilms and planktonic cultures in presence of epinephrine.

Gannesen A V AV Schelkunov M I MI Ziganshin R H RH Ovcharova M A MA Sukhacheva M V MV Makarova N E NE Mart'yanov S V SV Loginova N A NA Mosolova A M AM Diuvenji E V EV Nevolina E D ED Plakunov V K VK

AIMS microbiology 20240527 2

Transcriptomic and proteomic analysis were performed on 72 h biofilms of the acneic strain <i>Cutibacterium acnes</i> and planktonic cultures in the presence of epinephrine. Epinephrine predominantly downregulated genes associated with various transporter proteins. No correlation was found between proteomic and transcriptomic profiles. In control samples, the expression of 51 proteins differed between planktonic cultures and biofilms. Addition of 5 nM epinephrine reduced this number, and in the ...[more]

PMID: 38919714

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