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MEF2A interactome in striated muscle by an affinity-purification followed by LC-MS-MS

ABSTRACT: Myocyte Enhancer Factor 2 (MEF2) proteins are involved in multiple developmental, physiological, and pathological processes in vertebrates. Protein:protein interactions underlie the plethora of biological processes impacted by MEF2A, necessitating a detailed characterization of the MEF2A interactome. A nanobody based affinity-purification/mass spectrometry strategy was employed to achieve this goal. Specifically, the MEF2A protein complexes were captured from myogenic lysates using a GFP-tagged MEF2A protein immobilized with a GBP-nanobody followed by LC-MS/MS proteomic analysis to identify MEF2A interactors.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Myoblast, Muscle

SUBMITTER: Declan Williams

LAB HEAD: John C McDermott

PROVIDER: PXD050042 | Pride | 2024-02-27

REPOSITORIES: Pride

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			Action	DRS
	20190823_HFX_CDS_MiyakeT_DM_GFP_1.raw	Raw
	20190823_HFX_CDS_MiyakeT_DM_GFP_2.raw	Raw
	20190823_HFX_CDS_MiyakeT_DM_M2A_1.raw	Raw
	20190823_HFX_CDS_MiyakeT_DM_M2A_2.raw	Raw
	20190823_HFX_CDS_MiyakeT_DM_M2D_1.raw	Raw

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MEF2A interactome in striated muscle by an affinity-purification followed by LC-MS-MS

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