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Identification of novel KRT32-associated proteins in primary keratinocytes cells lysate by GST-KRT32 pulldown assays coupled with mass spectrometry.

ABSTRACT: GST-KRT32 and its interacting proteins in primary keratinocytes cells were enriched by pull-down using glutathione-Sepharose beads. Then, KRT32-interacting proteins were identified by mass spectrum. Proteins obtained in Pulldown were subjected to SDS-PAGE gel electrophoresis and silver staining. The SDS-PAGE gel was sent to the mass spectrometry platform of the Jingjie PTM Biolab for subsequent processing and mass spectrometry detection.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Peidian Shi

LAB HEAD: Peidian Shi

PROVIDER: PXD050141 | Pride | 2024-06-17

REPOSITORIES: Pride

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			Action	DRS
	XA07835GI_K_C_GST.raw	Raw
	XA07835GI_K_C_G_KTR32.raw	Raw
	checksum.txt	Txt
	search.zip	Other

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