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Mechanism of phage sensing and abortion by toxin-antitoxin-chaperone systems (HDX-MS)

ABSTRACT: Toxin-antitoxins (TAs) are prokaryotic two-gene systems comprised of a toxin neutralised by an antitoxin. Toxin-antitoxin-chaperone (TAC) systems additionally include a SecB-like chaperone that stabilises the antitoxin by recognising its chaperone addiction (ChAD) element. TACs have been shown to mediate antiphage defence, but the mechanisms of viral sensing and restriction are unexplored. Here we tried to find the epitope between SecB and gpV with HDX-MS.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Escherichia Coli Bw25113

SUBMITTER: kyo Coppieters

LAB HEAD: Chloé Martens

PROVIDER: PXD050182 | Pride | 2024-03-14

REPOSITORIES: Pride

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			Action	DRS
	231220_SecB_ref1.raw.zip	Raw
	231220_SecB_ref1_IA_final_peptide.csv	Csv
	231220_SecB_ref2.raw.zip	Raw
	231220_SecB_ref2_IA_final_peptide.csv	Csv
	231220_SecB_ref3.raw.zip	Raw

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