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G2 Phase Controls Binary Division of Toxoplasma gondii

ABSTRACT: The current study investigates the roles of a novel essential cell cycle kinase TgCrk4. We identified this kinase aalong with its cyclin partner and demonstrated that TgCrk4 regulates processes carried out during conventional G2 phase, such as repression of chromosome re-replication and centrosome re-duplication. Accumulation of TgCyc4 in the nucleus and on the centrosomes, supported the role of TgCrk4-TgCyc4 complex as a coordinator of chromosome and centrosome cycles in T. gondii.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Toxoplasma Gondii Me49

TISSUE(S): Cell Culture

SUBMITTER: Clem Marsilia

LAB HEAD: Elena Suvorova

PROVIDER: PXD050478 | Pride | 2024-06-23

REPOSITORIES: Pride

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	20230323_global_Crk4_0min-rep1-1.raw	Raw
	20230323_global_Crk4_0min-rep1-2.raw	Raw
	20230323_global_Crk4_0min-rep1-3.raw	Raw
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	20230323_global_Crk4_0min-rep1-5.raw	Raw

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Publications

The Crk4-Cyc4 complex regulates G<sub>2</sub>/M transition in Toxoplasma gondii.

Hawkins Lauren M LM Wang Chengqi C Chaput Dale D Batra Mrinalini M Marsilia Clem C Awshah Danya D Suvorova Elena S ES

The EMBO journal 20240410 11

A versatile division of apicomplexan parasites and a dearth of conserved regulators have hindered the progress of apicomplexan cell cycle studies. While most apicomplexans divide in a multinuclear fashion, Toxoplasma gondii tachyzoites divide in the traditional binary mode. We previously identified five Toxoplasma CDK-related kinases (Crk). Here, we investigated TgCrk4 and its cyclin partner TgCyc4. We demonstrated that TgCrk4 regulates conventional G<sub>2</sub> phase processes, such as repress ...[more]

PMID: 38600241

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