Synechocystis sp.PCC6803 LC-MS/MS

ABSTRACT: About 200 ml liquid culture (OD730 = 0.4) was centrifuged at 4 000 g, 4 ºC, for 10 min, and the supernatant containing the extracellular proteins was collected. The supernatant was filtered through 0.45 µm pore size filters to remove remaining cell debris, and the proteins in the filtrate were further concentrated by ultrafiltration using centrifugal filters with a molecular weight cut-off of 5 kDa (Millipore). The concentrated extracellular medium was precipitated with 10% trichloroacetic acid at 4 ºC overnight and then dissolved in 50 mM ammonium bicarbonate

INSTRUMENT(S): Q Exactive

ORGANISM(S): Synechocystis Sp. Pcc 6803

TISSUE(S): Cell Culture

SUBMITTER: wencan Zheng  

LAB HEAD: wencan zheng

PROVIDER: PXD050522 | Pride | 2024-05-21

REPOSITORIES: Pride