Proteomics

Dataset Information

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LC-MS of Craniofacial cartilage organoids from ESCs


ABSTRACT: Knowledge of cell signaling pathways that drive human neural crest differentiation into craniofacial chondrocytes is incomplete, yet essential for using stem cells to regenerate craniomaxillofacial structures. To accelerate translational progress, we developed a differentiation protocol that generated self-organizing craniofacial cartilage organoids from human embryonic stem cell-derived neural crest stem cells. Histological staining of cartilage organoids revealed tissue architecture and staining typical of elastic cartilage. Protein and post-translational modification (PTM) mass spectrometry and snRNASeq data showed that chondrocyte organoids expressed robust levels of cartilage extracellular matrix (ECM) components: many collagens, aggrecan, perlecan, proteoglycans, and elastic fibers. We identified two populations of chondroprogenitor cells, mesenchyme cells and nascent chondrocytes and the growth factors involved in paracrine signaling between them. We show that ECM components secreted by chondrocytes not only create a structurally resilient matrix that defines cartilage, but also play a pivotal autocrine cell signaling role to determine chondrocyte fate.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: TYLER LEVY  

LAB HEAD: Mark Grimes

PROVIDER: PXD050668 | Pride | 2024-04-04

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
030520_Foltz_AKT.raw Raw
030520_Foltz_ATM.raw Raw
030520_Foltz_pY.raw Raw
030620_Foltz_IMAC_OT_f1.raw Raw
030620_Foltz_IMAC_OT_f10.raw Raw
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