Dataset Information

Mouse Exercise & Sedentary Extracellular Vesicles

ABSTRACT: In our study, we conducted a comprehensive proteomic analysis on small extracellular vesicles (sEVs) and exercise-induced extracellular vesicles (eEVs) to investigate their potential roles and protein cargos that may be pertinent to neurodegenerative diseases, particularly Alzheimer's Disease (AD). We successfully identified 929 proteins within eEVs, with 84 proteins significantly enriched compared to sEVs. These proteins were predominantly associated with established EV markers, validating our EV isolation technique. However, there was limited correlation between these significantly different proteins in eEVs and existing human plasma and EV datasets, except when comparing overall protein profiles with those upregulated in human plasma after exercise, suggesting a unique exercise-induced protein signature in eEVs. Further pathway analysis revealed that proteins differentially expressed between eEVs and sEVs are involved in critical pathways such as mitochondrial biogenesis and integrin signaling. These findings are significant given the central role of dysfunctional mitochondria in AD and the potential of integrin-mediated interactions in directing EV tropism and facilitating neuronal uptake of EVs, which could be crucial for therapeutic interventions. Additionally, our results indicate an enrichment of Mitogen-Activated Protein Kinases (MAPKs) activation in eEVs, aligning with previous studies on the activation of MAPK in muscle regeneration and neuronal healing processes. This suggests that exercise-induced EVs may carry beneficial proteins for neuronal health and recovery, offering new insights into the mechanisms underlying the positive effects of exercise on neurodegenerative diseases. Further investigations are necessary to explore the specific roles of exercise-induced integrins in neuronal uptake and the therapeutic potential of eEVs in AD.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Plasma, Extracellular Vesicle

SUBMITTER: Oliver Fuller

LAB HEAD: Mark Anthony Febbraio

PROVIDER: PXD050696 | Pride | 2025-01-06

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	EV_DIA_Proteomics.csv	Csv
	HF2OF20221216_EV-PILOT_20221216115108.sld	Other
	HF2OF20221216_P21_0280_Exp5_E-1_20221217135555.raw	Raw
	HF2OF20221216_P21_0280_Exp5_E-2_20221217220149.raw	Raw
	HF2OF20221216_P21_0280_Exp5_S-1_20221217175855.raw	Raw

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Mouse Exercise & Sedentary Extracellular Vesicles

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