Proteomics

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Multi-Omic analyses reveal aberrant differentiation trajectory with WNT1 Loss-of-Function in type XV osteogenesis imperfecta


ABSTRACT: Osteogenesis imperfecta (OI) is a serious genetic bone disorder characterized by congenital low bone mass, deformity and frequent fractures. Type XV OI is a moderate to severe form of skeletal dysplasia caused by WNT1 mutations. In this cohort study from southern China, we summarized the clinical phenotypes of patients with WNT1 mutations and found the proportion of type XV patients was around 10.3% (25 out of 243) with diverse phenotypic spectrums. Functional assays indicated that mutations of WNT1 significantly impaired its secretion and effective activity, leading to moderate to severe clinical manifestations, porous bone structure and enhanced osteoclastic activities. Analysis of proteomic data from human skeleton indicated that the expression of SOST was dramatically reduced in type XV patients. Single-cell transcriptome data generated from human tibia samples revealed aberrant differentiation trajectory of skeletal progenitors and impaired maturation of osteocytes, resulting in excessive CXCL12+ progenitors and abnormal cell populations with adipogenic characteristics. The integration of multi-omics data from human skeleton delineates how WNT1 regulates the differentiation and maturation of skeletal progenitors, which will provide a new direction for the treatment strategy of type XV osteogenesis imperfecta and relative low bone mass diseases such as early onset osteoporosis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Osteoblast, Bone

DISEASE(S): Osteogenesis Imperfecta,Proteus Syndrome

SUBMITTER: Peikai Chen  

LAB HEAD: Michael Kai Tsun To

PROVIDER: PXD050841 | Pride | 2024-10-17

REPOSITORIES: Pride

Dataset's files

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Action DRS
22030801_SG_Control1.raw Raw
22030801_SG_Control2.raw Raw
22030801_SG_Control3.raw Raw
22030801_SG_Control4.raw Raw
22030801_SG_OI1.raw Raw
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Publications

Multi-omics analyses reveal aberrant differentiation trajectory with WNT1 loss-of-function in type XV osteogenesis imperfecta.

Tan Zhijia Z   Chen Peikai P   Zhang Jianan J   Shek Hiu Tung HT   Li Zeluan Z   Zhou Xinlin X   Zhou Yapeng Y   Yin Shijie S   Dong Lina L   Feng Lin L   Wong Janus Siu Him JSH   Gao Bo B   To Michael Kai Tsun MKT  

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 20240901 9


Osteogenesis imperfecta (OI) is a group of severe genetic bone disorders characterized by congenital low bone mass, deformity, and frequent fractures. Type XV OI is a moderate to severe form of skeletal dysplasia caused by WNT1 variants. In this cohort study from southern China, we summarized the clinical phenotypes of patients with WNT1 variants and found that the proportion of type XV patients was around 10.3% (25 out of 243) with a diverse spectrum of phenotypes. Functional assays indicated t  ...[more]

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