Proteomics

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Resistance target screening of ositinib-sensitive and resistant H1975 cell lines


ABSTRACT: (1) Extract and purify proteins from oxitinib-sensitive and resistant H1975 cell lines, screen for proteins exhibiting differences in stability using different denaturation conditions, and digest and digest them using proteases to obtain enzymatic products. (2) The enriched proteins were quantitatively analysed by liquid chromatography-mass spectrometry (LC-MS/MS) to screen for specific resistance targets.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Lung Cancer

SUBMITTER: jian wang  

LAB HEAD: Jian wang

PROVIDER: PXD051034 | Pride | 2024-07-09

REPOSITORIES: Pride

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