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NEDD8 loss causes cancer vulnerability to immune checkpoint blockade in triple-negative breast cancer

ABSTRACT: We have blocked the expression of some genes in triple-negative breast cancer cell line, MDA-MB-231, and we want to analyse the global protein changes in knockout (KO) versus control cells.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Irineos Papakyriacou

LAB HEAD: Yumeng Mao

PROVIDER: PXD051061 | Pride | 2024-06-23

REPOSITORIES: Pride

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	KO1.raw	Raw

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Publications

Loss of NEDD8 in cancer cells causes vulnerability to immune checkpoint blockade in triple-negative breast cancer.

Papakyriacou Irineos I Kutkaite Ginte G Rúbies Bedós Marta M Nagarajan Divya D Alford Liam P LP Menden Michael P MP Mao Yumeng Y

Nature communications 20240427 1

Immune checkpoint blockade therapy aims to activate the immune system to eliminate cancer cells. However, clinical benefits are only recorded in a subset of patients. Here, we leverage genome-wide CRISPR/Cas9 screens in a Tumor-Immune co-Culture System focusing on triple-negative breast cancer (TNBC). We reveal that NEDD8 loss in cancer cells causes a vulnerability to nivolumab (anti-PD-1). Genetic deletion of NEDD8 only delays cell division initially but cell proliferation is unaffected after r ...[more]

PMID: 38678024

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