Proteomics

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R-protein composition of budding yeast PTC deficient 80S ribosomes

ABSTRACT: Ribosomal RNA modifications are introduced during ribosome assembly by snoRNA and stand-alone modification enzyme based mechanisms in budding yeast. Lack of individual modifications in rRNA has a minor effect on yeast growth, ribosome biogenesis, and translation, which has complicated the definition of the biological function of rRNA modifications. We have constructed a S.cerevisiae strain lacking 7 25S rRNA modifications (Ψ2826, Um2921, Gm2619, m5C2870, Ψ2880, Um2724 and Gm2922)in peptidyl-transferase center. This strain exhibits severely compromised growth at 30°C, reduced level of global translation and actively translating ribosomes and cold sensitivity. Ribosomal protein composition of the mature 80S ribosomes was analyzed by SILAC based qMS approach. We have found that absence of PTC modifications around PTC does not affect significantly r-protein content of 80S ribosomes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Margus Leppik  

LAB HEAD: Tiina Tamm

PROVIDER: PXD051211 | Pride | 2024-06-23

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
170823_02_Orbi1_SK_SER_G120_T163_TYMRI_Remme_Kisly_309_80S.raw Raw
231123_03_Orbi3_MS_SER_G60_T85_Leppik_silac_309_906_500ng.raw Raw
231221_03_Orbi3_MS_SER_G60_T85_Leppik_silac_309_309_500ng.raw Raw
231221_05_Orbi3_MS_SER_G60_T85_Leppik_silac_309_906_500ng.raw Raw
Sample_description.txt Txt
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Publications

Loss of Conserved rRNA Modifications in the Peptidyl Transferase Center Leads to Diminished Protein Synthesis and Cell Growth in Budding Yeast.

Leppik Margus M   Pomerants Liisa L   Põldes Anett A   Mihkelson Piret P   Remme Jaanus J   Tamm Tiina T  

International journal of molecular sciences 20240510 10

Ribosomal RNAs (rRNAs) are extensively modified during the transcription and subsequent maturation. Three types of modifications, 2'-O-methylation of ribose moiety, pseudouridylation, and base modifications, are introduced either by a snoRNA-driven mechanism or by stand-alone enzymes. Modified nucleotides are clustered at the functionally important sites, including peptidyl transferase center (PTC). Therefore, it has been hypothesised that the modified nucleotides play an important role in ensur  ...[more]

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