Proteomics

Dataset Information

0

Metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions


ABSTRACT: Purple phototrophic bacteria (PPB) naturally accept CO2 into their metabolism as a primary redox sink system in photo-heterotrophy. Dedicated use of this feature for developing sustainable processes (e.g., through negative-emissions photo-bioelectrosynthesis) requires a deep knowledge of the inherent metabolic mechanisms. Here we provide evidence of the tuning of the PPB metabolic mechanisms upon redox stressing through negative polarization (-0.4 and -0.8 V vs. Ag/AgCl) in photo-bioelectrochemical devices. Using metaproteomic analysis at both reactor ans species level, we showed that a mixed PPB-culture up-regulates its ability to capture CO2 from organics oxidation through the Calvin-Besson-Bassam cycle and anaplerotic pathways, and the redox imbalance is promoted to polyhydroxyalkanoates production. The ecological relationship of PPB with mutualist bacteria stabilizes the system and opens the door for future development of photo-bioelectrochemical devices focused on CO2 up-cycling.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Rhodobacter Capsulatus

SUBMITTER: Baptiste Leroy  

LAB HEAD: Leroy Baptiste

PROVIDER: PXD051223 | Pride | 2024-10-29

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
0.4v-0.wiff Wiff
0.4v-0.wiff.scan Wiff
0.4v-1.wiff Wiff
0.4v-1.wiff.scan Wiff
0.4v-2.wiff Wiff
Items per page:
1 - 5 of 33

Similar Datasets

2020-09-09 | GSE157613 | GEO
2016-06-10 | GSE80158 | GEO
2022-07-12 | E-MTAB-11370 | biostudies-arrayexpress
2017-02-14 | PXD004963 | Pride
2016-06-10 | E-GEOD-80158 | biostudies-arrayexpress
2019-07-15 | PXD008838 | Pride
2013-12-20 | E-GEOD-53360 | biostudies-arrayexpress
2008-06-13 | E-GEOD-5639 | biostudies-arrayexpress
2022-05-20 | PXD024889 | Pride
2024-05-04 | PXD041920 | JPOST Repository