Dataset Information

Human SUMOylated Golgin45 CoIP-MS

ABSTRACT: HeLa cells expressing mCherry-Golgin45, Flag-UBC9-WT/DN and myc-SUMO1 were subjected to Co-IP experiment using RFP-beads (M165-8, MBL life science). Independent triplicates of mCherry-Golgin45 Co-IP experiments were prepared and analyzed by MS independently in a label free format.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Xihua Yue

LAB HEAD: Intaek Lee

PROVIDER: PXD051237 | Pride | 2024-06-23

REPOSITORIES: Pride

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Publications

SUMOylated Golgin45 associates with PML-NB to transcriptionally regulate lipid metabolism genes during heat shock stress.

Jing Shuaiyang S Gao Jingkai J Tiwari Neeraj N Du Yulei Y Zhu Lianhui L Gim Bopil B Qian Yi Y Yue Xihua X Lee Intaek I

Communications biology 20240506 1

Golgin tethers are known to mediate vesicular transport in the secretory pathway, whereas it is relatively unknown whether they may mediate cellular stress response within the cell. Here, we describe a cellular stress response during heat shock stress via SUMOylation of a Golgin tether, Golgin45. We found that Golgin45 is a SUMOylated Golgin via SUMO1 under steady state condition. Upon heat shock stress, the Golgin enters the nucleus by interacting with Importin-β2 and gets further modified by S ...[more]

PMID: 38710927

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