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Products differentially secreted from perigonadal adipose tissue of db/db mice.

ABSTRACT: A control group of male C57BL/6J mice were fed a 60% fat diet (Research Diets D12492i) for 8 weeks, resulting in “ad libitum obesity” (ALO). Leptin receptor-deficient B6.BKS(D)-Leprdb/J (“db/db”) male mice were fed a low-fat chow diet (PicoLab Rodent Diet 20; Purina Mills Inc). Once the average body weights of the two groups were equivalent, all mice were fasted for 24 hours, after which they were sacrificed and perigonadal adipose tissue (PGAT) was dissected out. PGAT was cultured ex vivo for 6 hours, after which conditioned media was collected and submitted for proteomic profiling. At the time of sacrifice, ALO mice were 17 weeks old and db/db mice were 10 weeks old. “HFD” is also used to refer to the ALO condition while “DB” is used to refer to the “db/db” condition.

INSTRUMENT(S): Bruker Daltonics timsTOF series

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Adipose Tissue

SUBMITTER: Rajesh Soni

LAB HEAD: Anthony Ferrante Jr.

PROVIDER: PXD051294 | Pride | 2025-02-23

REPOSITORIES: pride

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			Action	DRS
	JG1181.zip	Other
	JS1181_TimsTofPro_diaPASEF_Spectronaut_ProteinGroups_Report.xlsx	Xlsx

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