Proteomics

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Kinase-catalyzed Biotinylation for Discovery and Validation of Substrates to Multi-specificity Kinases NME1 and NME2


ABSTRACT: Protein phosphorylation by kinases regulates mammalian cell functions, such as growth, division, and signal transduction. Among human kinases, NME1 and NME2 are associated with metastatic tumor suppression, but remain understudied due to the lack of tools to monitor their cellular substrates. In particular, NME1 and NME2 are multi-specificity kinases phosphorylating serine, threonine, histidine, and aspartic acid residues of substrates, and the heat and acid sensitivity of phosphohistidine and phosphoaspartate complicates substrate discovery and validation. To provide new substrate monitoring tools, we established the γ-phosphate modified ATP analog, ATP-biotin, as a cosubstrate for phosphorylbiotinylation of NME1 and NME2 cellular substrates. Building upon this ATP-biotin compatibility, the Kinase-catalyzed Biotinylation with Inactivated Lysates for Discovery of Substrates (K-BILDS) method enabled validation of a known substrate and the discovery of seven NME1 and three NME2 substrates. Given the paucity of methods to study kinase substrates, ATP-biotin and the K-BILDS method are valuable tools to characterize the roles of NME1 and NME2 in human cell biology.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Eric Davis  

LAB HEAD: Mary K Pflum

PROVIDER: PXD051411 | Pride | 2024-10-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
2294_A_04.pdResultView Other
2294_A_101.mgf Mgf
2294_A_101.msf Msf
2294_A_101.msfView Msf
2294_A_101.mzML Mzml
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Publications

Kinase-catalyzed biotinylation for discovery and validation of substrates to multispecificity kinases NME1 and NME2.

Gary Chelsea R CR   Acharige Nuwan P N NPN   Oyewumi Tolulope O TO   Pflum Mary Kay H MKH  

The Journal of biological chemistry 20240718 8


Protein phosphorylation by kinases regulates mammalian cell functions, such as growth, division, and signal transduction. Among human kinases, NME1 and NME2 are associated with metastatic tumor suppression but remain understudied due to the lack of tools to monitor their cellular substrates. In particular, NME1 and NME2 are multispecificity kinases phosphorylating serine, threonine, histidine, and aspartic acid residues of substrate proteins, and the heat and acid sensitivity of phosphohistidine  ...[more]

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