Proteomics

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SILAC APMS analysis of SRP14 interactome in HEK-293 cells


ABSTRACT: The Signal Recognition Particle (SRP) is a universally conserved and abundant ribonucleoprotein particle (RNP) in cells. Its composition and size vary across evolution. In mammals, SRP comprises one RNA molecule, the 7SL RNA, and six proteins: SRP14, SRP14, SRP19, SRP54, SRP68, and SRP72. SRP is essential for targeting transmembrane and secreted proteins to the endoplasmic reticulum. To reveal novel aspects of SRP biogenesis, we established the interactome of the GFP-SRP72 protein. To do so, we have produced stable human U2OS cell line expressing GFP-SRP14 and performed stable isotope labeling by amino acids in cell culture (SILAC) proteomic experiment using GFP-SRP14 as bait.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293 Cell

SUBMITTER: Franck Vandermoere  

LAB HEAD: Dr Severine Massenet

PROVIDER: PXD051488 | Pride | 2024-06-07

REPOSITORIES: Pride

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