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Deep phosphoproteomics of Klebsiella pneumoniae reveals HipA-mediated tolerance to ciprofloxacin

ABSTRACT: Klebsiella pneumoniae belongs to the group of bacterial pathogens causing the majority of antibiotic-resistant nosocomial infections worldwide; however, the molecular mechanisms underlying post-translational regulation of its physiology are poorly understood. Here we perform a comprehensive analysis of Klebsiella phosphoproteome, focusing on HipA, a Ser/Thr kinase involved in antibiotic tolerance in Escherichia coli. We show that overproduced K. pneumoniae HipA (HipAkp) is toxic to both E. coli and K. pneumoniae and its toxicity can be rescued by overproduction of the antitoxin HipBkp. Importantly, HipAkp overproduction leads to increased tolerance against ciprofloxacin, a commonly used antibiotic in the treatment of K. pneumoniae infections. Proteome and phosphoproteome analyses in the absence and presence of ciprofloxacin confirm that HipAkp has Ser/Thr kinase activity, auto-phosphorylates at S150, and shares multiple substrates with HipAec, thereby providing a valuable resource to clarify the molecular basis of tolerance and the role of Ser/Thr phosphorylation in this human pathogen.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Escherichia Coli Klebsiella Pneumoniae Subsp. Pneumoniae

SUBMITTER: Payal Nashier

LAB HEAD: Boris Macek

PROVIDER: PXD051521 | Pride | 2024-11-21

REPOSITORIES: Pride

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			Action	DRS
	20210210_PN_HipAkp_Rep1_Chymo_Proteome.raw	Raw
	20210210_PN_HipAkp_Rep1_Chymo_phospho1.raw	Raw
	20210210_PN_HipAkp_Rep1_Chymo_phospho2.raw	Raw
	20210210_PN_HipAkp_Rep1_Chymo_phospho3.raw	Raw
	20210210_PN_HipAkp_Rep1_Chymo_phospho4.raw	Raw

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