Proteomics

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Opportunistic pathogen Porphyromonas gingivalis targets the LC3B-ceramide complex and mediates lethal mitophagy resistance in oral tumors

ABSTRACT: Mechanisms by which Porphyromonas gingivalis (P. gingivalis) infection enhances oral tumor growth or resistance to cell death remain elusive. Here, we determined that P. gingivalis infection mediates therapeutic resistance via inhibiting lethal mitophagy in cancer cells and tumors. Mechanistically, P. gingivalis targets the LC3B-ceramide complex by associating with LC3B via bacterial major fimbriae (FimA) protein, preventing ceramide-dependent mitophagy in response to various therapeutic agents. Moreover, ceramide-mediated mitophagy is induced by Annexin A2 (ANXA2)-ceramide association, involving the E142 residue of ANXA2. Inhibition of ANXA2-ceramide-LC3B complex formation by wild-type P. gingivalis prevented ceramide-dependent mitophagy. Moreover, a FimA-deletion mutant P. gingivalis variant had no inhibitory effects on ceramide-dependent mitophagy. Further, 16S rRNA sequencing of oral tumors indicated that P. gingivalis infection altered the microbiome of the tumor macroenvironment in response to ceramide analog treatment in mice. Thus, these data provide a mechanism describing the pro-survival roles of P. gingivalis in oral tumors.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Porphyromonas Gingivalis

SUBMITTER: Jennifer Bethard  

LAB HEAD: Besim Ogretmen

PROVIDER: PXD051568 | Pride | 2024-06-23

REPOSITORIES: Pride

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Action DRS
020420_SP_HUMAN-n20354.fasta Fasta
04152020_Ogretmen_1.msf Msf
04152020_Ogretmen_1_20200417210106.msf Msf
04152020_Ogretmen_1_20200417210106.raw Raw
04152020_Ogretmen_2-01.msf Msf
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Opportunistic pathogen <i>Porphyromonas gingivalis</i> targets the LC3B-ceramide complex and mediates lethal mitophagy resistance in oral tumors.

Sheridan Megan M   Chowdhury Nityananda N   Wellslager Bridgette B   Oleinik Natalia N   Kassir Mohamed Faisal MF   Lee Han G HG   Engevik Mindy M   Peterson Yuri Y   Pandruvada Subramanya S   Szulc Zdzislaw M ZM   Yilmaz Özlem Ö   Ogretmen Besim B  

iScience 20240430 6

Mechanisms by which <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) infection enhances oral tumor growth or resistance to cell death remain elusive. Here, we determined that <i>P. gingivalis</i> infection mediates therapeutic resistance via inhibiting lethal mitophagy in cancer cells and tumors. Mechanistically, <i>P. gingivalis</i> targets the LC3B-ceramide complex by associating with LC3B via bacterial major fimbriae (FimA) protein, preventing ceramide-dependent mitophagy in response to  ...[more]

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