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Impact of 5-FU on the proteome of HEK293T cells

ABSTRACT: In this study, we wanted to examine the effects of 5-fluorouracil, a drug used in anticancer treatment, on the proteome of HEK293T cells (human cell culture). HEK293T cells were exposed to 5-fluorouracil (5-FU) using two different incubation strategies. In the first experiment, cells were incubated in 5-FU supplied medium and harvested after 24h acute exposure. The second experiment included a 24h exposure to 5-FU, followed by a recovery phase, were cells were incubated for another 6h in fresh medium without 5-FU. We performed shotgun proteomics of the respective tryptic digest of both experiments and compared the proteome of treated and untreated cells, respectively.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Maximilian Berg

LAB HEAD: Stefanie Kaiser

PROVIDER: PXD051672 | Pride | 2025-03-18

REPOSITORIES: Pride

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			Action	DRS
	20240207_53_c1.raw	Raw
	20240207_53_c2.raw	Raw
	20240207_53_c3.raw	Raw
	20240207_53_c5.raw	Raw
	20240207_53_c6.raw	Raw

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Publications

NAIL-MS reveals tRNA and rRNA hypomodification as a consequence of 5-fluorouracil treatment.

Berg Maximilian M Li Chengkang C Kaiser Stefanie S

Nucleic acids research 20250201 4

Recent studies have investigated RNA modifications in response to stressors like chemical agents, including the anticancer drug 5-fluorouracil (5-FU). Traditionally, 5-FU's mechanism of action was believed to involve inhibition of thymidylate synthase, leading to thymidine depletion and cancer cell death. However, recent findings suggest that ribosome collisions and defects in ribosomal RNA (rRNA) processing drive 5-FU toxicity, potentially through RNA writer inhibition. To explore the effects o ...[more]

PMID: 39997220

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