Monitoring post-translational modifications and associated proteomes of WT-PML-V and two mutants from acute promyelocytic leukaemia patients refractory for arsenic therapy: A216T-PML-V and L217F-PML-V

ABSTRACT: U2OS PML-/- cells were reconstituted with YFP-PML-V of three types: WT, A216T or L217F. These cell lines were used to purify YFP-PML bodies using anti-GFP nanobody beads from cells either treated or not with arsenic (1uM for 2h). Protein samples were monitored for PML post-translational modifications and associated proteomes to try to understand why people with acute promyelocytic leukaemia that is resistant to arsenic treatment have these mutations.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Mike Tatham  

LAB HEAD: Ronald T. Hay

PROVIDER: PXD051743 | Pride | 2025-03-27

REPOSITORIES: Pride