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Genetic excision of the regulatory cardiac troponin I extension in high heart rate mammal clades

ABSTRACT: Mammalian cardiac troponin I (cTnI) contains a highly conserved N-terminal extension harboring protein kinase A targets (Ser23/24) which are phosphorylated during ß-adrenergic stimulation to increase cardiomyocyte relaxation rate. Here, we show that the Ser23/24 encoding exon 3 of TNNI3 was pseudoexonized multiple times in shrews and moles to mimic Ser23/24 phosphorylation without adrenergic stimulation, thereby facilitating the evolution of exceptionally high resting heart rates (~1000 beats min-1). We further reveal alternative splicing of TNNI3 exon 3 in distantly related bat families with both cTnI isoforms being incorporated into cardiac myofibrils.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Hipposideros Armiger Suncus Murinus

TISSUE(S): Heart

SUBMITTER: mengdie zhang

LAB HEAD: Kai He

PROVIDER: PXD051845 | Pride | 2024-07-19

REPOSITORIES: Pride

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			Action	DRS
	20240415-ZMD-Bat-22kd.mgf	Mgf
	20240415-ZMD-Bat-22kd.mzid.gz	Mzid
	20240415-ZMD-Bat-22kd.raw	Raw
	20240415-ZMD-Bat-29kd.mgf	Mgf
	20240415-ZMD-Bat-29kd.mzid.gz	Mzid

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Genetic excision of the regulatory cardiac troponin I extension in high heart rate mammal clades

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