Dataset Information

DLG1 functions upstream of SDCCAG3 and IFT20 to control ciliary targeting of polycystin-2

ABSTRACT: Polarized vesicular trafficking directs specific receptors and ion channels to cilia, but the underlying mechanisms are poorly understood. Here we describe a role for DLG1, a core component of the Scribble polarity complex, in regulating ciliary protein trafficking in kidney epithelial cells. Conditional knockout of Dlg1 in mouse kidney caused causes ciliary elongation and cystogenesis, and cell-based proximity labelling proteomics and fluorescence microscopy showed alterations in the ciliary proteome upon loss of DLG1. Specifically, the retromer-associated protein SDCCAG3, IFT20 and polycystin-2 (PC2) were are reduced in cilia of DLG1 deficient cells compared to control cells. This phenotype was is recapitulated in vivo and rescuable by re-expression of wildtype DLG1, but not a Congenital Anomalies of the Kidney and Urinary Tract (CAKUT)-associated DLG1 variant, p.T489R. Finally, biochemical approaches and Alpha Fold modelling suggested that SDCCAG3 and IFT20 form a complex that associates, at least indirectly, with DLG1. Our work identifies a key role for DLG1 in regulating ciliary protein composition and suggests that ciliary dysfunction of the p.T489R DLG1 variant may contribute to CAKUT.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Karsten Boldt

LAB HEAD: Dr. Karsten Boldt

PROVIDER: PXD051912 | Pride | 2024-05-14

REPOSITORIES: pride

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			Action	DRS
	JUN3659-S02-A-C1_cilia_BIOID2_WT_1.raw	Raw
	JUN3659-S03-A-C7_cilia_BIOID2_DLG1KO_1.raw	Raw
	JUN3659-S04-A-C25_DLG1_BIOID2_1.raw	Raw
	JUN3659-S05-A-C13_BIOID2_WT_1.raw	Raw
	JUN3659-S06-A-C19_BIOID2_DLG1KO_1.raw	Raw

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DLG1 functions upstream of SDCCAG3 and IFT20 to control ciliary targeting of polycystin-2

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