Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ACL1-HD-Zip IV complex revealed common mechanism in rice response to brown planthopper and drought. Being a small peptide, ACL1 might function through forming complex with other proteins. So that we searched the possible interacting proteins of ACL1 using the TurboID system.

ABSTRACT: Brown planthopper (BPH) is the most notorious insect pest to rice. Drought is the most commonly occurring global adversity. BPH infestation caused adaxially-rolled leaves and shrunk bulliform cells similar to drought. The bulliform-cell characteristic gene, ACL1, negatively regulated BPH resistance and drought tolerance, with decreased cuticular wax in ACL1-D, which resulted in quicker water losing. ACL1 was specifically expressed in epidermis. TurboID system and various biochemical assays revealed that ACL1 interacted with the epidermal-characteristic HD-Zip IV ROCs. ROC4 and ROC5 positively regulated BPH resistance and drought tolerance through modulating cuticular wax and bulliform cells respectively. Overexpression of ROC4 and ROC5 both rescued ACL1-D in various related phenotypes simultaneously. Moreover, ACL1 competed with ROC4 and ROC5 in homo-dimerization and hetero-dimerization. Altogether, we illustrated that ACL1-ROCs complex synergistically mediate drought tolerance and BPH resistance through regulating cuticular wax and bulliform cells in rice, a new mechanism which might facilitate BPH resistance breeding.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Oryza Sativa (rice)

SUBMITTER: Zhihuan Tao

LAB HEAD: Zhihuan Tao

PROVIDER: PXD052228 | Pride | 2024-08-13

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20240122DDA60MIN_ZY_RICE_Acl1_Slot1-21_1_4017.d.rar	Other
	20240122DDA60MIN_ZY_RICE_ZH11_Slot1-19_1_4011.d.rar	Other
	20240122DDA60MIN_ZY_RICE_turbo_Slot1-18_1_4008.d.rar	Other
	20240320TZH_census_labelfree_out_20572_stat.txt	Txt
	checksum.txt	Txt

Items per page:

1 - 5 of 5

Similar Datasets

β-diketone accumulation in modern bread wheat varieties

Project description:Cuticular waxes coating leaf surfaces can help tolerate drought events by reducing non-stomatal water loss. Despite their role in drought tolerance, little is known about the cuticular wax responses of Canadian bread wheat varieties. To fill in this gap, RNAseq was performed on the flag leaf of four modern varieties to identify potential markers that could be used for selection of higher accumulation of cuticular waxes. This analysis revealed that the W1 locus is a good candidate for higher accumulation of β-diketones.

2024-08-01 | GSE254797 | GEO

Rice plants infested with planthoppers

2007-08-21 | GSE8811 | GEO

Gene expression data of OsHMGB707 overexpression and RNAi transgenic rice plants under normal conditions

Project description:High mobility group (HMG) proteins play an important role in regulation of gene transcription through modulate the structure of DNA. In this study, OsHMGB707, a HMG gene localized in rice drought resistance QTL interval, was isolated and the function on rice stress resistance was identified. Overexpression of OsHMGB707 significantly enhanced the drought resistance of the transgenic rice plants, whereas the OsHMGB707-RNAi transgenic rice plants exhibited slightly decrease in drought stress tolerance. To search the downstream genes regulated by OsHMGB707, we performed microarray analysis of the OsHMGB707-overexpressing, OsHMGB707-RNAi and wild-type plants under both normal conditions using Affymetrix Rice Genome Genechip. 21-day-old plants of the OsHMGB707-overexpressing line OE1, OsHMGB707-RNAi line RNAi1 as well as the wild-type plants were used in the normal condition.

2019-05-08 | GSE130821 | GEO

Changes in rice NILs root expression profile in response to drought stress at reproductive stage

Project description:The current knowledge of drought stress transcriptomes in rice are mostly relying on comparative studies of diverse genetic background under drought. A more reliable approach is to use near-isogenic lines (NILs) with a common genetic background (Donar parent: Aday Sel, Recurrent parent: IR64), but contrasting levels of resistance to drought stress under initial exposure to water deficit. Here, we examined two pairs of NILs in IR64 background with contrasting drought tolerance. We obtained gene expression profile in roots of rice NILs under different levels of drought stress help to identify genes and mechanisms involved in drought stress.

2012-07-01 | GSE30463 | GEO

Changes in rice NILs panicle expression profile in response to drought stress at reproductive stage

Project description:The current knowledge of drought stress transcriptomes in rice are mostly relying on comparative studies of diverse genetic background under drought. A more reliable approach is to use near-isogenic lines (NILs) with a common genetic background (Donar parent: Aday Sel, Recurrent parent: IR64), but contrasting levels of resistance to drought stress under initial exposure to water deficit. Here, we examined two pairs of NILs in IR64 background with contrasting drought tolerance. We obtained gene expression profile in panicles of rice NILs under different levels of drought stress help to identify genes and mechanisms involved in drought stress.

2012-07-01 | GSE30462 | GEO

Changes in rice NILs shoot expression profile in response to drought stress at reproductive stage

Project description:The current knowledge of drought stress transcriptomes in rice are mostly relying on comparative studies of diverse genetic background under drought. A more reliable approach is to use near-isogenic lines (NILs) with a common genetic background (Donar parent: Aday Sel, Recurrent parent: IR64), but contrasting levels of resistance to drought stress under initial exposure to water deficit. Here, we examined two pairs of NILs in IR64 background with contrasting drought tolerance. We obtained gene expression profile in shoots of rice NILs under different levels of drought stress help to identify genes and mechanisms involved in drought stress.

2012-07-01 | GSE30449 | GEO

A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative resistance in rice

Project description:Transcription factors play a crucial regulatory role in plant drought stress responses. In this study, a novel drought stress related bZIP transcription factor, OsbZIP62, was identified in rice. This gene was selected from transcriptome analysis of several typical rice varieties with different drought tolerance. The expression of OsbZIP62 was obviously induced by drought, hydrogen peroxide, and abscisic acid (ABA) treatment. Overexpression of OsbZIP62-VP64 (OsbZIP62V) enhanced the drought tolerance and oxidative stress tolerance of transgenic rice, while the osbzip62 mutants showed the opposite phenotype. RNA-seq analysis showed that many stress-related genes (e.g. OsGL1, OsNAC10, and DSM2) were up-regulated in OsbZIP62V plants. OsbZIP62 could bind to the abscisic acid–responsive element (ABRE) and promoters of several putative target genes. Taken together, OsbZIP62 positively regulated rice drought tolerance through regulated the expression of genes associated with stress.

2018-11-28 | GSE122887 | GEO

Transcription profiling of rice plants infested with planthoppers

Project description:Infestation with white-backed planthopper (WBPH) to rice caused induced resistance to rice pathogens but brown planthopper (BPH) infestation induce weaker resistance to rice pathogens. We compared changes in gene expression in rice plants infested with WBPH and BPH to gain some insight into the WBPH-induced resistance to rice pathogens. An analysis, using microarrays, of gene expression in rice plants infested with these planthoppers revealed that WBPH infestation caused high induction of many defense-related genes including pathogenesis-related (PR) genes than BPH infestation. Furthermore, hydroperoxide lyase 2 (OsHPL2) which is an enzyme to produce C6 volatiles was induced by WBPH infestation, but not by BPH infestation. Experiment Overall Design: Agilent rice oligo microarray was used to investigate the gene expression profiling in rice plants infested with WBPH or BPH. Total RNA was extracted from pooled leaf blades infested with WBPH or BPH for 24 h and from mock-treated pooled leaf blades. Total RNA (200 ng) was labeled with Cy-3 or Cy-5 using an Agilent low RNA input linear amplification kit. Fluorescently labeled targets were hybridized to Agilent rice oligo microarrays. Hybridization and wash processes were performed according to the manufacturerâ??s instructions, and hybridized microarrays were scanned using an Agilent DNA microarray scanner. Agilent Feature Extraction software was employed for the image analysis and data extraction processes. Fold changes in expression level in each treatment were compared with those of the respective mock-treated controls. In each treatment, the experiment was performed independently three times.

2008-06-16 | E-GEOD-8811 | biostudies-arrayexpress

Transcript profiling of drought responsiveness in two contrasting rice genotypes during vegetative stage stress

2012-05-01 | GSE5978 | GEO

Transcript profiling of drought responsiveness in two contrasting rice genotypes during vegetative stage stress

Project description:Rice (Oryza sativa), the major staple food crop is being cultivated under varying ecosystems ranging from irrigated lowland to rainfed upland environments. Improvement in the rice production under drought prone unfavourable environment depends on the development of drought tolerant genotypes which needs thorough understanding of physiological and molecular events behind the tolerance mechanism. There is considerable genetic variation for drought tolerance mechanism within the cultivated gene pool. To understand the diversity of drought response, two indica rice genotypes namely, i) Apo, an up-land drought tolerant indica veriety from Philippines and ii) IR64, a popular high yielding drought susceptible genotype were selected for this study. We used the 22K rice Oligoarray from Agilent technologies to study the transcript profile in the leaves of the two contrasting rice genotypes under control and drought stressed conditions during vegetative phase. Keywords: Drought response We used Agilent rice gene chips (G4138A) to investigate the transcript level changes in rice leaf tissues during drought stress. We used two contrasting rice genotypes (IR64 drought susceptible and Apo drought tolerant) differing in their degree of drought tolerance. Plants were grown under green house conditions and drought stress was imposed on 33rd DAS. Leaf sampling was done in both control and drought stressed plants after 6 days of drought stress. Three replications of microarray experiments were carried out by hybridizing the control samples against the drought stressed samples.

2012-04-30 | E-GEOD-5978 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data